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Table 1 Relationship between floral morphological characteristics and female meiotic stage of P. pseudo-simonii × P. nigra ‘Zheyin3#’

From: Induction of unreduced megaspores with high temperature during megasporogenesis in Populus

Days of culture

Female floral stage

Floral morphological characteristics

Representative female meiotic stages

9.5

I

Bud is tightly wrapped by bract scales

MMC-early leptotene

10.5

II

Slight bulginess in the mid bud region

MMC-late leptotene

13

III

Bract scales dehisced slightly, catkin not emerged

Leptotene-diakinesis

13.5

IV

Bract scales dehisced and catkin slightly emerged

Pachytene-prophase II

15

V

One third of the catkin outside of bract scales

Diakinesis-telophase II

15.5

VI

Half of the catkin outside of bract scales

Prophase II-tetrad

16.5

VII

Two third of the catkin outside of bract scales

Metaphase II-uninucleate embryo sac

  1. MMC megaspore mother cell