Table 2 Triploid production by high-temperature-induced megaspore chromosome doubling in P. pseudo-simonii × P. nigra ‘Zheyin3#’
From: Induction of unreduced megaspores with high temperature during megasporogenesis in Populus
Female floral stage | Treatment temperature (°C) | Treatment duration (h) | Seed number | Seedling number | Triploid number | Triploid production rate (%) |
|---|---|---|---|---|---|---|
Stage II | 38 | 3 | 69 | 17 | 0 | 0 |
5 | 36 | 11 | 0 | 0 | ||
41 | 3 | 6 | 2 | 0 | 0 | |
5 | 28 | 13 | 1 | 7.69 | ||
44 | 3 | – | – | – | – | |
5 | – | – | – | – | ||
Stage III | 38 | 3 | 122 | 0 | – | – |
5 | 107 | 26 | 0 | 0 | ||
41 | 3 | 42 | 3 | 2 | 66.67 | |
5 | 112 | 19 | 7 | 36.84 | ||
44 | 3 | – | – | – | – | |
5 | – | – | – | – | ||
Stage IV | 38 | 3 | 108 | 27 | 0 | 0 |
5 | 232 | 87 | 10 | 11.49 | ||
41 | 3 | 173 | 110 | 24 | 21.82 | |
5 | 198 | 124 | 26 | 20.97 | ||
44 | 3 | 87 | 55 | 20 | 36.36 | |
5 | 81 | 25 | 15 | 60.00 | ||
Stage V | 38 | 3 | 149 | 13 | 0 | 0 |
5 | 150 | 22 | 3 | 13.64 | ||
41 | 3 | 188 | 60 | 2 | 3.33 | |
5 | 97 | 14 | 2 | 14.29 | ||
44 | 3 | 161 | 10 | 3 | 30.00 | |
5 | 209 | 22 | 8 | 36.36 | ||
Stage VI | 38 | 3 | 251 | 109 | 1 | 0.92 |
5 | 175 | 57 | 3 | 5.26 | ||
41 | 3 | 111 | 47 | 3 | 6.38 | |
5 | 91 | 46 | 5 | 10.87 | ||
44 | 3 | 143 | 51 | 5 | 9.80 | |
5 | 127 | 39 | 6 | 15.38 | ||
Control | 479 | 127 | 0 | 0 | ||
Total | 3,732 | 1,136 | 146 |