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Table 2 ANOVA of the effect of storage time (0, 12 months), storage temperature (10°, −3°, −20 °C, −196 °C) and water content on P. nigra seed germination. Seeds had a WC of 0.15 g g−1 (non-desiccated) or 0.07 g g −1 (desiccated). ANOVA mixed model with “tree” as a random effect. Seeds from harvest in 2011. WC Water content

From: Optimal seed water content and storage temperature for preservation of Populus nigra L. germplasm

Source variance

DF

DF Den

F

P

ST

1

2

65.04

0.0115

STE

3

6

66.26

0.0001

Seed WC

1

2

10.42

0.084

ST*STE

3

6

71.45

0.0001

ST*WC

1

2

10.31

0.0848

STE*WC

3

6

9.87

0.0098

ST*STE*WC

3

6

7.74

0.0174