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Fig. 5 | Annals of Forest Science

Fig. 5

From: Certainties and uncertainties about the life cycle of the Périgord black truffle (Tuber melanosporum Vittad.)

Fig. 5

Dikaryotic tissue, karyogamy, meiosis and ascospore differentiation in non-mature ascogenous tissue of T. melanosporum ascocarps (a, b dilacerated ascocarp stained with 1 μM DAPI and Calcofluor White Stain (Invitrogen) imaged with Zeiss LSM 780 confocal microscope at 40× magnification. c–e 30-μm thick cut of T. melanosporum ascocarp stained with 1 μM DAPI and observed under UV fluorescence with an Olympus BX42TF epifluorescence microscope at 63× magnification). a Ascogenous tissue with dikaryotic cells. b Young ascus mother cells with two nuclei and then one nucleus after karyogamy. c Meiose in ascus mother cells. d Individualization of cytoplasm and cell wall around the two haploid nuclei just after the meiotic division in ascus mother cells. e Ascogenous tissue with plurinucleate ascospores in ascus mother cell

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Annals of Forest Science

ISSN: 1297-966X