Effect of seed storage temperature on fine root development and mycorrhizal colonization of young Populus nigra seedlings

Table 2 Two-way ANOVA of the effect of seed treatment (3 °C, −3 °C, −10 °C, −20 °C and cryopreserved at −196 °C for 24 months and freshly harvested) and root order on anatomical traits of seedling root. Seeds to experimental design came from tree No. 1

	% of roots with primary development		Root diameter (μm)		% cortex in root diameter		Nitrogen content (% of d.m.^a)
	F	P	F	P	F	P	F	P
Treatment	9.08	<0.001	2.60	0.025	18.12	<0.001	6.39	<0.001
Root order	102.26	<0.001	120.51	<0.001	156.64	<0.001	59.85	<0.001
Treatment × root order	2.38	0.021	2.26	0.005	4.91	<0.001	0.58	0.860

ISSN: 1297-966X