Fig. 2
From: Molecular marker associated with a deleterious recessive anomaly in Eucalyptus grandis seedlings
a Polymorphic marker (arrow) obtained with primer AD17. The marker occurs in all abnormal individuals (left) and in some normal individuals (right). b Schematic of the marker presence in the genitors and full-sib progeny that presents three classes NNM (normal without marker), NM (normal with marker), and AM (abnormal with marker). c Sequence characterized amplified region (SCAR) marker validation. The marker is shown in all abnormal individuals (right) and in two normal individuals (recombinants) (left). G07 and G26 are the genitors. L (a, c): 1 kb ladder (Invitrogen, Carlsbad, CA). d SCAR [AnoDel, (Anomaly and Deleterious)] sequence shows the two-base polymorphism (red). e Southern blot analysis showing the presence of more than one copy of SCAR marker in the genome of genitors. f Diagram showing alignment between AnoDel, Phytozome EST, and GenBank EST sequence analyses. Similarity between the three sequences (red). g Diagram of marker sequence alignment with the eucalypt genome