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Fig. 5 | Annals of Forest Science

Fig. 5

From: Short-day photoperiods affect expression of genes related to dormancy and freezing tolerance in Norway spruce seedlings

Fig. 5

Gene expression profiles of LN2 (a, b), LN3 (c, d), and LN4 (e, f) used for indicating dormancy induction. The level of gene expression is given as delta delta threshold cycle (ddCt), which is the relative difference between the gene of interest and reference genes (genes that did not show any significant difference in expression). SD (short day) treatments (7, 14, 21, and 28 days) with a photoperiod of 11 h started on July 15. Measurements were performed on July 15 (day 0), July 22 (day 7), July 29 (day 14), Aug. 5 (day 21), and Aug. 12 (day 28) and on Aug. 26 (day 42). After termination of SD treatment, the Norway spruce seedlings were either kept indoors (a, c, e) or moved outdoors (b, d, f). Each sampling day, eight apical buds were collected from each SD treatment and growing environment and then analyzed for gene expression as one general sample

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Annals of Forest Science

ISSN: 1297-966X