Fig. 6

ColdNSure™ freezing tolerance phases (adapted from Balk et al. 2007) at different dates of sampling (Sept. 9, Sept. 23, Oct. 9, Oct. 23) for control and SD (short day) treated (11 h light; 13 h night) seedlings from both indoor and outdoor growth environments. ColdNSure™ phase 0 = cold sensitive; the indicator profiles match the profiles of lots that are actively growing and no sign of freezing tolerance could be recognized. Phase 1 = developing freezing tolerance; early signs of freezing tolerance development can be recognized. Phase 2 = developing freezing tolerance; tolerance level approaches full freezing tolerance. Phase 3 = freezing tolerant; the indicator profiles match the profiles of lots that have ceased growth and that are fully tolerant, ready for lifting and storage. Each sampling date, eight apical buds were collected from the controls and each SD treatment from both growing environments and then analyzed for gene expression as one general sample