Table 2 The sequences and amplification of 14 ISSR primers
The number of primer | Primer sequence (5′-3′) | Annealing temperature (°C) | Total number of loci | Number of polymorphic loci | Percentage of polymorphic loci (%) |
|---|---|---|---|---|---|
UBC808 | AGA GAG AGA GAG AGA GC | 54.6 | 18 | 15 | 83.3 |
UBC811 | GAG AGA GAG AGA GAG AC | 54.6 | 15 | 12 | 80.0 |
UBC817 | CAC ACA CAC ACA CAC AA | 52.2 | 7 | 5 | 71.4 |
UBC825 | ACA CAC ACA CAC ACA CT | 52.2 | 12 | 8 | 66.7 |
UBC827 | ACA CAC ACA CAC ACA CG | 54.6 | 9 | 4 | 44.4 |
UBC828 | TGT GTG TGT GTG TGT GA | 52.2 | 12 | 7 | 58.3 |
UBC834 | AGA GAG AGA GAG AGA GYT | 53.9 | 16 | 13 | 81.3 |
UBC835 | AGA GAG AGA GAG AGA GYC | 56.2 | 11 | 9 | 81.8 |
UBC836 | AGA GAG AGA GAG AGA GYA | 53.9 | 15 | 8 | 53.3 |
UBC840 | GAG AGA GAG AGA GAG AYT | 53.9 | 14 | 12 | 85.7 |
UBC841 | GAG AGA GAG AGA GAG AYC | 56.2 | 15 | 5 | 33.3 |
UBC842 | GAG AGA GAG AGA GAG AYG | 56.2 | 13 | 8 | 61.5 |
UBC848 | CAC ACA CAC ACA CAC ARG | 56.2 | 13 | 10 | 76.9 |
UBC849 | GTG TGT GTG TGT GTG TYA | 53.9 | 10 | 7 | 70.0 |
Total | – | – | 180 | 123 | 68.3 |