Table 1 Species contributions to the 13 pool types used to test the pooling strategy and accuracy of predictions of numbers of hybrid individuals in each pool type using SSR markers across 4 replicates. The species composition of the 13 pool types is shown as well as their expected mean peak ratio (q value, see “Materials and methods” for full explanation) and the q value range that was used to assign hybrid number. A. auriculiformis is abbreviated as AA, A. mangium as AM and AH as acacia hybrid (AM x AA)
Pool type | Ratio of species-specific alleles | Expected mean peak ratio for A. mangium–specific alleles | Peak ratio range (q value) for assigning no. of hybrid seedlings | Number of replications (pools) | Number of pools successfully predicted | No. of pools under-predicted | No. of pools over-predicted |
|---|---|---|---|---|---|---|---|
10AA | 20AA:0AM | 0 | 0–0.004 | 4 | 4 | ||
9AA:1AH | 19AA:1AM | 0.05 | 0.005–0.074 | 4 | 3 | 1 | |
8AA:2AH | 18AA:2AM | 0.10 | 0.075–0.124 | 4 | 2 | 2 | |
7AA:3AH | 17AA:3AM | 0.15 | 0.125–0.174 | 4 | 3 | 1 | |
6AA:4AH | 16 AA:4AM | 0.20 | 0.175–0.224 | 4 | 2 | 2 | |
5AA:5AH | 15AA:5AM | 0.25 | 0.225–0.274 | 4 | 2 | 1 | 1 |
10AH | 10AA:10AM | 0.50 | 0.475–0.524 | 4 | 3 | 1 | |
5AM:5AH | 5AA:15AM | 0.75 | 0.725–0.774 | 4 | 4 | ||
6AM:4AH | 4AA:16AM | 0.80 | 0.775–0.824 | 4 | 2 | 2 | 0 |
7AM:3AH | 3AA:17AM | 0.85 | 0.825–0.874 | 4 | 1 | 3 | |
8AM:2AH | 2AA:18AM | 0.90 | 0.875–0.924 | 4 | 2 | 2 | |
9AM:1AH | 1AA:19AM | 0.95 | 0.925–0.995 | 4 | 3 | 1 | |
10AM | 0AA:20AM | 1 | 0.996–1.0 | 4 | 4 | ||
Total | 52 | 31 | 4 | 17 |