Clinal variation along precipitation gradients in Patagonian temperate forests: unravelling demographic and selection signatures in three Nothofagus spp.

Table 1 Population genetic diversity and differentiation of each species and microsatellite molecular markers employed (EST-SSR = expressed sequence tags-transcriptome; gSSR = genome)

Species	Pop	ID	Precip. Mm/year	gSSRs				EST-SSRs
Species	Pop	ID	Precip. Mm/year	N	A_R	H_E	F_ST/G”_ST	N	A_R	H_E	F_ST/G”_ST
Nothofagus nervosa (Lácar Watershed)	Bandurrias	N1	1400	30.44	27.0	0.430^A	0.061/ 0.118	34.75	11.8	0.383 ^B	0.022/0.039
	Quila Quina	N2	1600	32	32.7	0.469 ^A		23	13.9	0.463 ^B
	Quilanlahue	N3	2000	27.6	34.9	0.453 ^A		29	12.3	0.399 ^B
	Yuco	N4	1900	26.11	34.3	0.477 ^A		29	11.4	0.406^B
	Quechuquina	N5	2400	19.89	36.6	0.492 ^A		25	12.9	0.509 ^A
	Cerro Malo	N6	2400	36.89	34.6	0.439 ^A		42	12.4	0.506 ^AB
	HuaHum	N7	2500	44.56	34.3	0.501 ^A		46	12.6	0.493 ^AB
	*Mean values			31.07	33.5	0.466		32.68	12.5	0.451
Nothofagus obliqua (Lácar Watershed)	Bandurrias	O1	1400	30.78	33	0.497 ^A	0.092/0.156	33.75	12.6	0.507 ^A	0.051/0.071
	Quila Quina	O2	1600	31.78	30.5	0.486 ^A		30.5	14.0	0.495 ^A
	Quilanlahue	O3	2000	27.78	33.3	0.504 ^A		28	11.5	0.514 ^A
	Yuco	O4	1900	33.56	27.4	0.473 ^A		35	9.6	0.481 ^A
	Nonthué	O5	2450	35.11	33.1	0.469 ^A		38	10.9	0.476 ^A
	HuaHum	O6	2500	17.78	32.5	0.495 ^A		20	11	0.502 ^A
	*Mean values			29.47	31.6	0.487		30.88	11.6	0.496
Nothofagus pumilio (Vintter Watershed)	J. S. Martín	P1	200	27.14	35.0	0.537 ^A	0.037/0.059	18.75	28.2	0.470 ^A	0.019/0.001
	C. Lago Guacho	P2	1000	27.14	41.6	0.569 ^A		19	24.3	0.419 ^A
	Lago Guacho	P3	1400	57.29	43.2	0.584 ^A
	Falso Engaño	P4	1000	26	42.0	0.560 ^A
	Lago Engaño	P5	1800	37.71	38.4	0.561 ^A		17.25	27.9	0.456 ^A
	Río Pico	P6	800	23.86	38.2	0.536 ^A
	*Mean values			33.19	39.7	0.558		18.33	26.8	0.448

Pop sampled population, N average number of individuals per population, H_E Nei’s unbiased expected heterozygosity (different letters within species indicate significant differences between populations, after Friedman test for multiple comparisons), A_R allelic richness, F_ST population differentiation coefficient, G”_ST standardized population differentiation coefficient. *Means are only comparable between N. nervosa and N. obliqua because they were analysed with the same loci

ISSN: 1297-966X