Table 3 nSSR and EST-SSR loci, respective primer sequences, repeat motif, reference and labelling
Multiplex | SSR locus | Type | Primer sequences (5′–3′) | Repeat motif | Reference | Labelling |
|---|---|---|---|---|---|---|
Primer mix 1 | SPAG7.14 | nSSR | F:TTCGTAGGACTAAAAATGTGTG R:CAAAGTGGATTTTGACCG | (TG)17 (AG)21 | Soranzo et al. (1998) | VIC |
pn6360 | nSSR | F:CAACTTCCTTCACCTGGCAC R:GACCCTTCTCAGCATCAACAC | (TC)20 | Giovannelli et al. (2017) | 6-FAM | |
pn7754 | nSSR | F:TCAAGCTAATGCTGGGAACTC R:GTTGGTCGTGGTGACAAAGG | (TA)12 | 6-FAM | ||
pn2153 | nSSR | F:TTGTGGGCTTAACTCGTCAG R:TGGCATTTGCTTCTCATTTG | (TG)12 | VIC | ||
pn4379 | nSSR | F:ACAGGCACACAATTTGGTCC R:CATCATCTGCAGAAGGGAAGAC | (AT)15 | VIC | ||
pn2246 | nSSR | F:TCATGTCCAGCTTTGGTTGC R:AAGGTTCATTTCCACTCCGG | (TA)13 | PET | ||
pn6175 | nSSR | F:ATTTCCCGCCTACCATTACC R:GTTACCTGCAATTCGTGTGG | (AT)12 | NED | ||
pn1403 | nSSR | F:AGGCAATGATCATGTGGGTC R:GGATAGCCTGCAACTCCAATG | (GA)13 | NED | ||
Primer mix 2 | PHA_6062 | EST-SSRs | F:CTCTCTTTCTCTTCGGTACTCA R:AAGTTTCAGAGGATTCACACTC | (TGC)8 | Leonarduzzi et al. (2016b) | 6-FAM |
PHA_4783 | EST-SSRs | F:AATCTTCTCTTTCTTTGACTGTGT R:CATGGGAATGTTAGGAGTGC | (TCC)8 | VIC | ||
pn8747 | nSSR | F:GCATTCTTTCCTTGCTCTCG R:CTCATCTGCTCCCTCAGTCC | (AT)12 | Giovannelli et al. (2017) | VIC | |
NED | ||||||
pn6266 | nSSR | F:TGCCTTGCTGATTTATTTCCG R:CACTTTCAGCTACGACCGAC | (GT)14 | NED | ||
PET | ||||||
PtTX4001 | nSSR | F:CTATTTGAGTTAAGAAGGGAGTC R:CTGTGGGTAGCATCATC | (GT)15 | Zhou et al. (2002) | VIC | |
PtTX3107 | nSSR | F:AAACAAGCCCACATCGTCAATC R:TCCCCTGGATCTGAGGA | (CAT)14 | Elsik and Williams (2001) | PET |