First evidence for allotriploid hybrids between Juniperus thurifera and J. sabina in a sympatric area in the French Alps

Table 4 Primer sequences and PCR annealing temperatures. F: Forward, R: Reverse

Primer F	Primer F sequence (5′–> 3′)	Primer R	Primer R sequence (5′–> 3′)	Annealing temperature
ITSA	GGAAGGAGAAGTCGTAACAAGG	ITSB	CTTTTCCTCCGCTTATTGATATG	61 °C
petN	AACGAAGCGAAAATCAATCA	psbM	AAAGAGAGGGATTCGTATGGA	57 °C
trnL	CGAAATCGGTAGACGCTACG	trnF	ATTTGAACTGGTGACACGAG	60 °C
trnS	GCCGCTTTAGTCCACTCAGC	trnG	GAACGAATCACACTTTTACCAC	62 °C
trnD	ACCAATTGAACTACAATCCC	trnT	CTACCACTGAGTTAAAAGGG	60 °C

ISSN: 1297-966X