Table 6 Adapters and primers used for the AFLP experiment
Name | Step of AFLP experiment | Sequence (5′–> 3′) |
|---|---|---|
EcoRI L adapter | Ligation | CTCGTAGACTGCGTACC |
EcoRI S adapter | Ligation | AATTGGTACGCAGTC |
MseI L adapter | Ligation | GACGATGAGTCCTGAG |
MseI S adapter | Ligation | TACTCAGGACTCAT |
EcoRI primer | Pre-amplification | ACTGCGTACCAATTCA |
MseI primer | Pre-amplification | GATGAGTCCTGAGTAAC |
EcoRI primer 1 | Selective amplification (primer combination 1) | ACTGCGTACCAATTCACT |
MseI primer 1 | Selective amplification (primer combination 1) | GATGAGTCCTGAGTAACTA |
EcoRI primer 2 | Selective amplification (primer combination 2) | ACTGCGTACCAATTCACG |
MseI primer 2 | Selective amplification (primer combination 2) | GATGAGTCCTGAGTAACTT |