Two main genetic clusters with high admixture between forest and cultivated chestnut (Castanea sativa Mill.) in France

Bouffartigue, Cathy; Debille, Sandrine; Fabreguettes, Olivier; Cabrer, Ana Ramos; Pereira-Lorenzo, Santiago; Flutre, Timothée; Harvengt, Luc

doi:10.1007/s13595-020-00982-w

Annals of Forest Science

Table 2 SSRs markers and multiplex

From: Two main genetic clusters with high admixture between forest and cultivated chestnut (Castanea sativa Mill.) in France

Multiplex (set, dye)	Locus	Primer forward	Primer reverse	Linkage group (LG)	Annealing temperature (Ta)
1, VIC	EMCs14	GTGCTTCAGGGACCTTTCTTCTC	GCCGCCGCCTCCTGCTGCTC	5	57 °C
1, FAM	EMCs15	CTCTTAGACTCCTTCGCCAATC	CAGAATCAAAGAAGAGAAAGGTC	9	57 °C
1, FAM	EMCs38	TTTCCCTATTTCTAGTTTGTGATG	ATGGCGCTTTGGATGAAC	4	57 °C
1, NED	EMCs2^a	GCTGATATGGCAATGCTTTTCCTC	GCCCTCCAGCCTCACCTTCATCAG	6	57 °C
1, PET	CsCAT14	CGAGGTTGTTGTTCATCATTAC	GATCTCAAGTCAAAAGGTGTC	2	57 °C
1, VIC	CsCAT2^b	GTAACTTGAAGCAGTGTGAAC	CGCATCATAGTGAGTGACAG	10	57 °C
2, PET	CsCAT16^a	CTCCTTGACTTTGAAGTTGC	CTGATCGAGAGTAATAAAG	6	50 °C
2, FAM	CsCAT41^{b, d}	AAGTCAGCCAACACCATATGC	CCCACTGTTCATGAGTTTCT	8	50 °C
2, PET	QpZAG110	GGAGGCTTCCTTCAACCTACT	GATCTCTTGTGTGCTGTATTT	7	50 °C
2, VIC	QpZAG36	GATCAAAATTTGGAATATTAAGAGAG	ACTGTGGTGGTGAGTCTAACATGTAG	1	50 °C
2, NED	CsCAT3	CACTATTTTATCATGGACGG	CGAATTGAGAGTTCATACTC	12	50 °C
3, NED	QrZAG4^c	CGTCTATAAGTTCTTGGGTGA	GTAACTATGATGTGATTCTTACTTCA	3	48 °C
3, NED	QrZAG96^c	CCCAGTCACATCCACTACTGTCC	GGTTGGGAAAAGGAGATCAGA	10	52 °C
4, Ned	CsCAT6	AGTGCTCGTGGTCAGTGAG	CAACTCTGCATGATAAC	1	50 °C
4, PET	CsCAT1	GAGAATGCCCACTTTTGCA	GCTCCCTTATGGTCTCG	8	50 °C
4, FAM	CsCAT15	TTCTGCGACCTCGAAACCGA	GCTAGGGTTTTCATTTCTAG	8	50 °C
4, VIC	CsCAT8	CTGCAAGACAAGAATTACAC	GAATAACCTGCAGAAGGC	6	50 °C
5, FAM	RIC	AAGACAGAGACAGTGGTTTTTGC	TCTGGGGAAACACGAAGC	–	58 °C
5, PET	CsCAT17	TTGGCTATACTTGTTCTGCAAG	GCCCCATGTTTTCTTCCATGG	2	58 °C
5, VIC	EMCs22	GTGCCTCTGTATGCATGGTAAGC	CCAGGTTTAAGAAAGCAAGCATAAC	2	58 °C
6, FAM	EMCs25	ATGGGAAAATGGGTAAAGCAGTAA	AACCGGAGATAGGATTGAACAGAA	–	60 °C
6, NED	CIO	TCTGGGGAAACACGAAGC	TATTCCCATTCTGTCCCAAACAT	–	60 °C
6, PET	OCI	GGAATAAGTGGGGTGGGTTT	GGGCCAAAGCATCACATTAC	–	60 °C
6, VIC	OAL	CAATCTGAAAAGGTAATAGCCAGT	CCCAGGACATAAAATAGAAGCTG	–	60 °C

^aSSRs belonging to the same linkage group separated over 50 cm and therefore considered as being unlinked
^bLoci with null alleles
^cPost-PCR multiplexing
^dAmplification in 2 loci called CsCAT41A and CsCAT41 B (Pereira Lorenzo et al. 2010)

Back to article page

ISSN: 1297-966X

Contact us

Submission enquiries: annforsci@inrae.fr
General enquiries: info@biomedcentral.com