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Table 3 Effects of the thinning treatment (T) and the tree’s competitive status (CS, dominant or suppressed) on foliar secondary metabolites. Significantly different groups (UT, un-thinned; C, corridor thinning; CC, cross-corridor thinning) are distinguished with different letters (where ‘a’ is the group with the greatest mean concentration), based on Tukey’s test

From: Secondary metabolites in leaves of hybrid aspen are affected by the competitive status and early thinning in dense coppices

Compound

Model

Factor

Dominant trees

Suppressed trees

  

T

CS

T × CS

UT

C

CC

UT

C

CC

Dihydrochalcones

  Trihydroxy-methoxydihydrochalcone-O-glucoside 1

***

ns

***

ns

b

b

b

a

a

a

  Trihydroxy-methoxydihydrochalcone-O-glucoside 2

***

ns

***

*

c

c

c

bc

ab

a

  Hesperetin dihydrochalcone-O-glucoside 1

***

*

***

***

b

b

b

b

a

a

  Hesperetin dihydrochalcone-O-glucoside 2

***

ns

***

***

b

b

b

b

a

a

Flavonoids

  Catechin and epicatechin

***

***

***

***

ab

a

ab

c

b

ab

  (Epi)gallocatechin

***

***

***

*

a

a

a

b

a

a

  Procyanidin B type

***

***

***

***

ab

a

a

c

b

a

  Quercetin-O-pentoside 1

***

**

***

***

abc

a

ab

d

bc

c

  Quercetin-O-pentoside 2

***

**

***

***

ab

a

a

c

b

ab

  Kaempferol-3-O-galactoside

***

**

***

***

ab

a

ab

c

b

b

  Kaempferol-3-O-glucoside

***

*

***

***

ab

a

ab

c

b

ab

  Myricetin-O-pentoside

***

**

***

**

ab

a

a

d

c

bc

  Kaempferol-3-O-glucuronide

***

*

*

***

ab

a

bc

c

a

ab

  Quercetin-3-O-galacatoside

***

*

***

***

ab

a

ab

d

bc

c

  Quercetin-3-O-glucoside

***

*

***

***

ab

a

ab

c

b

ab

  Quercetin-3-O-glucuronide

***

**

***

***

ab

a

b

c

ab

ab

  Myricetin-3-O-galactoside

ns

-

-

-

-

-

-

-

-

-

  Myricetin-3-O-glucoside

***

***

***

**

abc

ab

a

d

cd

bc

  Myricetin-3-O-glucuronide

***

***

***

***

ab

a

ab

c

b

ab

  Kaempferol-O-pentosylhexoside

***

ns

**

***

ab

ab

bc

c

ab

a

  Kaempferol-O-rhamnosylhexoside

***

ns

ns

***

a

ab

ab

b

ab

ab

  Quercetin-3-O-arabinoglucoside

***

*

***

***

a

a

a

b

a

a

  Unk flavonoid 1

***

***

***

***

ab

a

bc

c

b

bc

  Unk flavonoid 2

ns

-

-

-

-

-

-

-

-

-

Hydroxycinnamates

  5-p-O-coumaroylquinic acid

***

ns

ns

***

ab

ab

bc

c

ab

a

  3-p-O-coumaroylquinic acid

***

**

***

***

a

a

a

b

a

a

  cis 3-O-caffeoylquinic acid

***

ns

***

**

a

a

ab

b

ab

ab

  trans 3-O-caffeoylquinic acid

***

ns

***

***

a

a

a

b

a

a

  5-O-caffeoylquinic acid

***

*

***

***

a

a

a

b

a

a

  4-O-caffeoylquinic acid

***

ns

***

***

ab

ac

ab

cd

bd

abcd

Jasmonates

  Hydroxyl-dihydrojasmonic acids

***

ns

***

ns

a

a

a

b

b

b

  Hydroxyjasmonyl sulfate

***

ns

***

*

b

ab

ab

a

ab

ab

  Unidentified jasmonate

ns

-

-

-

-

-

-

-

-

-

  Hydroxyjasmonic acid glucoside

***

ns

***

ns

b

b

b

a

a

a

Salicylates

  Salicin

***

ns

***

ns

b

b

b

a

a

a

  Salicortin

***

ns

***

ns

b

b

b

a

a

a

  Grandidentatin 1

***

***

***

**

c

bc

bc

b

a

a

  Grandidentatin 2

***

*

***

***

c

bc

bc

b

a

a

  Tremuloidin FA adduct

***

**

***

ns

c

d

cd

a

b

ab

  Salicortin FA adduct

***

ns

***

ns

b

b

b

a

a

a

  Tremulacin

***

ns

***

ns

b

b

b

a

a

a

Unknowns

  Unk1

ns

-

-

-

-

-

-

-

-

-

  Unk2

***

ns

***

ns

b

b

b

a

a

a

  1. Model—significance of the LMM model after Bonferroni correction for multiple testing (n = 43). ns not significant
  2. *p < 0.05; **p < 0.01; ***p < 0.001