Table 5 Retention times (tR), mass-to-charge ratios (m/z) and mass differences of compounds detected in methanolic extracts of hybrid aspen leaves
|  | tR (min) | Monoisotopic m/z [M-H]− | Observed m/z [M-H]− | Mass difference (ppm) |
|---|---|---|---|---|
Dihydrochalcones | Â | Â | Â | Â |
  Hesperetin dihydrochalcone-O-glucoside 1 | 41.4 | 465.1402 | 465.1392 | − 2.1 |
  Hesperetin dihydrochalcone-O-glucoside 2 | 44.4 | 465.1402 | 465.1392 | − 2.1 |
  Trihydroxy-methoxydihydrochalcone-O-glucoside 1 | 53.9 | 449.1453 | 449.1453 | 0.0 |
  Trihydroxy-methoxydihydrochalcone-O-glucoside 2 | 57.1 | 449.1453 | 449.1453 | 0.0 |
Flavonoids | Â | Â | Â | Â |
  (Epi)gallocatechin | 5.9 | 305.0667 | 305.0665 | − 0.7 |
  Procyanidin B type | 13.6 | 577.1351 | 577.1339 | − 2.1 |
  Catechin and epicatechin | 14.3 | 289.0718 | 289.0718 | 0.0 |
  Unk flavonoid 1 | 26.8 |  | 623.1254 |  |
  Myricetin-3-O-glucuronide | 29.1 | 493.0624 | 493.0639 | 3.0 |
  Myricetin-3-O- galactoside | 29.3 | 479.0831 | 479.0845 | 3.1 |
  Myricetin-3-O-glucoside | 30.0 | 479.0831 | 479.0850 | 4.0 |
  Unk flavonoid 2 | 31.2 |  | 623.1254 |  |
  Quercetin-3-O-arabinoglucoside | 32.2 | 595.1305 | 595.1327 | 3.8 |
  Myricetin-O-pentoside | 33.0 | 449.0725 | 449.0730 | 1.2 |
  Quercetin-3-O-galacatoside | 34.2 | 463.0882 | 463.0895 | 2.8 |
  Quercetin-3-O-glucuronide | 34.9 | 477.0675 | 477.0696 | 4.4 |
  Quercetin-3-O-glucoside | 35.5 | 463.0882 | 463.0894 | 2.6 |
  Kaempferol-O-rhamnosylhexoside | 36.4 | 593.1512 | 593.1533 | 3.5 |
  Kaempferol-O-pentosylhexoside | 36.6 | 579.1355 | 579.1380 | 4.3 |
  Quercetin-O-pentoside 1 | 37.5 | 433.0776 | 433.0786 | 2.3 |
  Kaempferol-3-O-galactoside | 38.0 | 447.0933 | 447.0950 | 3.8 |
  Quercetin-O-pentoside 2 | 38.8 | 433.0776 | 433.0789 | 3.0 |
  Kaempferol-3-O-glucuronide | 39.8 | 461.0725 | 461.0730 | 1.1 |
  Kaempferol-3-O-glucoside | 40.1 | 447.0933 | 447.0942 | 2.0 |
Hydroxycinnamates | Â | Â | Â | Â |
  cis 3-O-caffeoylquinic acid | 8.8 | 353.0878 | 353.0883 | 1.3 |
  trans 3-O-caffeoylquinic acid | 9.6 | 353.0878 | 353.0867 | − 3.1 |
  5-p-O-coumaroylquinic acid | 12.3 | 337.0929 | 337.0923 | − 1.8 |
  3-p-O-coumaroylquinic acid | 13.2 | 337.0929 | 337.0917 | − 3.6 |
  5-O-caffeoylquinic acid | 16.5 | 353.0878 | 353.0872 | − 1.7 |
  4-O-caffeoylquinic acid | 18.1 | 353.0878 | 353.0871 | − 2.0 |
Jasmonates | Â | Â | Â | Â |
  Hydroxyjasmonyl sulfate | 18.7 | 305.0700 | 305.0695 | − 1.6 |
  Hydroxyjasmonic acid glucoside | 23.3 | 387.1661 | 387.1669 | 2.1 |
  Unidentified jasmonate | 43.6 | 373.1868 | 373.1879 | 2.9 |
  Hydroxyl-dihydrojasmonic acid 1 | 63.3 | 227.1289 | 227.1297 | 3.5 |
  Hydroxyl-dihydrojasmonic acid 2 | 66.4 | 227.1289 | 227.1293 | 1.8 |
Salicylates | Â | Â | Â | Â |
  Salicin | 8.5 | 331.1035 | 331.1046 | 3.3 |
  Salicortin | 31.2 | 423.1297 | 423.1315 | 4.3 |
  Salicortin FA adduct | 31.3 | 469.1351 | 469.1372 | 4.5 |
  Grandidentatin 1 | 45.7 | 423.1661 | 423.1679 | 4.3 |
  Tremuloidin FA adduct | 48.4 | 435.1297 | 435.1316 | 4.4 |
  Grandidentatin 2 | 49.4 | 423.1661 | 423.1678 | 4.0 |
  Tremulacin | 64.9 | 527.1559 | 527.1584 | 4.7 |
Unknown compounds | Â | Â | Â | Â |
  Unk1 | 41.9 |  | 477.1038 |  |
  Unk2 | 51.7 |  | 465.1391 |  |