Table 2 Primers used in this study
From: Growth adaptability and foreign gene stability of TaLEA transgenic Populus simonii × nigra
Primer name | Primer sequence(5′ → 3′) | Expected amplification length(bp) | Detected lines |
|---|---|---|---|
XL-1 Chr01 | F: AGCTATGCAATCCCAACAACA | 737 bp | XL-1 |
R: TCGCAAGACCCTTCCTCTATA | |||
XL-1 Chr03 | F:GCCAAATGTTTGAACGATCGG | 751 bp | |
R:CTTGAAAGTTTTTGCCATTCCCT | |||
XL-1 Chr05 | F:GAAGAATTCCATAGCCTCGACA | 608 bp | |
R:GGAACAACACTCAACCCTATCTC | |||
XL-1 Chr09 | F:ATGGATTTGGGTACGGTGAAG | 729 bp | |
R:GTCATAGCCGAATAGCCTCTC | |||
XL-1 Chr14 | F:TGGAGATATCACATCAATCCACTTG | 756 bp | |
R:ACGCGCATAGCTAGTCTTTATTC | |||
XL-1 Chr16 | F:ATCTCCAGGAAATCAAATACCTTCC | 744 bp | |
R:CTTTCGTAGATCAGTTGCCTCAT | |||
XL-7 Chr09 | F:TTGCTGGTCTTGTTCTGTTCA | 723 bp | XL-7 |
R:ATTTAGTGCTTTACGGCACCT | |||
XL-9 Chr05 | F:CGGTTAAGAATTGAATTTCATAAT | 643 bp | XL-9 |
R:GTTACTAGATCGGGAATTCACTGG |