Low effective population size and high spatial genetic structure of black poplar populations from the Oder valley in Poland

Table 2 Measures of genetic diversity per population of black poplar based on 12 polymorphic nSSR loci. Population acronyms as in Fig. 1A

Pop.	N_MLL	A	Ae	A_R19	P_A	Ho	He	Fis	Fis_null	CI_Fisnull	Ne^	CI_Ne^	MR	Mreq
KK	48	11.17	5.16	8.86	3	0.67	0.76	0.09*	0.012nb	0.000 - 0.036	52.7	43.8 - 65.2	0.62*	0.81
B	42	10.25	5.64	8.57	5	0.72	0.79	0.09*	0.007nb	0.000 - 0.022	37.6	31.3 - 46.2	0.52*	0.80
WR	28	9.33	5.60	8.63	2	0.72	0.79	0.09*	0.008nb	0.000 - 0.028	25.4	20.5 - 32.3	0.57*	0.78
BD	77	12.58	6.25	9.29	5	0.70	0.80	0.12*	0.013nb	0.000 - 0.037	30.5	27.5 - 33.9	0.59*	0.82
CI	52	11.67	6.13	9.34	3	0.75	0.80	0.06*	0.011nb	0.000 - 0.035	133.5	95.4 - 211.6	0.60*	0.81
BO	34	9.92	5.44	8.71	2	0.71	0.78	0.09*	0.008nb	0.000 - 0.028	34.4	28.0 - 43.4	0.49*	0.79
CG	74	11.67	5.89	8.81	5	0.78	0.81	0.01	0.009nb	0.000 - 0.023	56.3	49.4 - 65.1	0.64*	0.83
K	19	6.67	4.21	6.67	3	0.66	0.71	0.07*	0.024nb	0.000 - 0.074	9.3	7.2 - 12.1	0.45*	0.78
G	29	6.67	4.07	6.20	2	0.70	0.71	0.01	0.006nb	0.000 - 0.023	5.2	3.4 - 6.4	0.47*	0.81
Mean	44.77	9.99	5.38	8.34	3.33	0.71	0.77	0.07	0.01		42.76		0.55	0.80

N_MLL, number of analyzed multilocus lineages (MLL); A, mean number of alleles; Ae, mean number of effective alleles; A_R19, allelic richness for a minimum sample size of 19 individuals; P_A, number of private alleles; Ho, observed heterozygosity; He, unbiased expected heterozygosity; Fis, multilocus inbreeding coefficient (*p < 0.001); Fis_Null, multilocus inbreeding coefficient with a correction for the presence of the null alleles (nb—random mating model (Fis = 0) was more probable than the full model; nfb—full model (Fis > 0) was more probable than random mating model), CI_FisNull and CI_Ne^, Bayesian 95% confidence intervals; Ne^, effective population size (Pcrit.= 0.02); Wilcoxon’s Test, signed-rank test under the T.P.M. model; MR, M-ratio (*—MR significantly < Mreq at p < 0.01); Mreq, mean MRs under mutation-drift equilibrium

ISSN: 1297-966X