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Pedigree and mating system analyses in a western larch (Larix occidentalis Nutt.) experimental population

Analyse de paternité et du mode de croisement dans une population expérimentale de mélèze occidental (Larix occidentalis Nutt.)

Annals of Forest Science volume 65, page 705 (2008)Cite this article

Abstract

  • •The mating pattern and gene flow in a western larch (Larix occidentalis Nutt.) experimental population was studied with the aid of microsatellite markers and a combination of paternity-mating system analysis. The commonly difficult to assess, male gametic contribution was determined with 95% confidence and its impact on genetic gain and diversity was determined.

  • • Male fertility success rate ranged between 0 and 11%. Male reproductive output parental imbalance was observed with 50% of the pollen being produced by the top 5% of males while the lower 39% males only produced 10% of the pollen.

  • • A significant difference was observed between male effective population size (genetic diversity) estimates from paternity assignment compared to those based on population’s census number (21 vs. 41); however, this difference did not affect estimates of genetic gain.

  • • A total of 221 full-fib families were identified (sample size range: 1–8) and were nested among the studied 14 seed-donors.

  • • A combination of paternity-mating system analysis is recommended to provide a better insight into seed orchards’ mating dynamics. While pollen flow tends to inflate mating system’s outcrossing rate, the paternity analysis effectively determined the rate and magnitude of contamination across receptive females.

Résumé

  • • Les modes de croisement et les flux de gènes dans une population expérimentale de mélèze occidental (Larix occidentalis Nutt.) ont été étudiés à l’aide de marqueurs microsatellites et d’une analyse combinée de paternité et du système de reproduction. La contribution gamétique mâle — communément difficile à estimer — a été déterminée avec un seuil de confiance de 95 % et son impact sur le gain génétique et la diversité a été déterminé.

  • • Le taux de succès reproductif mâle était compris entre 0 et 11 %. Un déséquilibre dans la contribution des parents mâles a été observé avec la production de 50 % du pollen par 5 % des pères alors que 39 % d’entre eux ne contribuaient que pour seulement 10 % du pollen.

  • • Une différence significative a été observée entre la taille efficace de la population mâle (diversité génétique) estimée par la recherche de paternité et celle basée sur les effectifs recensés de la population (21 vs. 41) ; cependant, cette différence n’affecte pas l’estimation du gain génétique.

  • • 221 familles de plein-frères ont été identifiées (effectifs entre 1 et 8), regroupées parmi les 14 arbres-mères étudiés.

  • • La combinaison d’une analyse de paternité et du système de reproduction est recommandée pour étudier de manière approfondie la dynamique de croisement en vergers à graines. Tandis que les flux de pollen tendent à augmenter le taux d’inter-croisements, l’analyse de paternité détermine de manière effective le taux et l’amplitude de contamination des arbres-mères.

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Authors and Affiliations

  1. Department of Forest Sciences, University of British Columbia, 2424 Main Mall, V6T 1Z4, Vancouver, British Columbia, Canada

    Tomas Funda, Charles C. Chen, Cherdsak Liewlaksaneeyanawin, Ahmed M. A. Kenawy & Yousry A. El-Kassaby

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  1. Tomas Funda
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  2. Charles C. Chen
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  3. Cherdsak Liewlaksaneeyanawin
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  4. Ahmed M. A. Kenawy
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  5. Yousry A. El-Kassaby
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Correspondence to Yousry A. El-Kassaby.

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Funda, T., Chen, C.C., Liewlaksaneeyanawin, C. et al. Pedigree and mating system analyses in a western larch (Larix occidentalis Nutt.) experimental population. Ann. For. Sci. 65, 705 (2008). https://doi.org/10.1051/forest:2008055

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Annals of Forest Science

ISSN: 1297-966X