The rooting ability of in vitro shoot cultures established from a UK collection of the common ash (Fraxinus excelsior L.) and their ex vitro survival

Table 2 Sets of primers and probes designed for real-time PCR identification and quantification of the contaminating microorganism

Target for amplification	Name	Primer/Probe	Oligonucleotides sequences
16S	BmBar_1F	Forward	5′-AGCCAATCCCATAAAACCAT-3′
	BmBar_1R	Reverse	5′-TAGCTCCTATACGGTTACTC-3′
	BmBar_1P	Probe	5′-CTCGCCTACATGAAGCTGGAATC-3′
	BmBar_2F	Forward	5′-GTCAAGCCAATCCCATAAAACCAT-3′
	BmBar_2R	Reverse	5′-GGCTAGCTCCTATACGGTTACTC-3′
	BmBar_2P	Probe	5′-TCAGTTCGGATTGTAGGCTGCAACTCG-3′
recA	recA_BmBar_1F	Forward	5′-TCCTGTATATGCTCAAAAATTAGG-3′
	recA_BmBar_1R	Reverse	5′-GCTCCAGATAGTTTACGCAA-3′
	recA_BmBar_1P	Probe	5′-TTAGTGCCAAAAGCGGAAATTGAAGGA-3′
	recA_BmBar_2F	Forward	5′-AGATCCTGTATATGCTCAAAAATTAGG-3′
	recA_BmBar_2R	Reverse	5′-GATAGCTCCAGATAGTTTACGCAA-3′
	recA_BmBar_2P	Probe	5′-TTAGTGCCAAAAGCGGAAATTGAAGGAGA-3′

The first column shows the reference numbers of the ash mother trees in the Earth Trust’s clonal archive of British and Irish ash trees, which provided the seeds used in this study. The second column shows the number of individual clonal ash shoot lines per half-sib family that were tested for their ability to root under in vitro conditions. The last column shows the in vitro rooting trials in which these clones were tested. The number of individual clones tested varied between 7 and 12 per family in most cases, depending on the availability of material. Note that the in vitro ash shoot clones used in trials 3, 4, 5, 6, and 7 were affected by contamination, and so they are not included here

ISSN: 1297-966X