Electrophysiological and behavioural responses of Ips typographus (L.) to trans-4-thujanol—a host tree volatile compound

Abstract

Key message

Spruce trees emit significant amounts of trans-4-thujanol, but the amount of this compound in bark decreases with tree age. Trans-4-thujanol acts as an efficient repellent for the bark beetle Ips typographus.

Context

The spruce bark beetle, Ips typographus, is an economically significant pest of Norway spruce (Picea abies) that preferentially infests and kills old trees.

Aims

We looked for spruce volatiles that were perceivable by I. typographus and that differed as the host tree aged.

Methods

Bark beetles were collected in the wild. Bark samples from spruce trees of different age were hydrodistilled. Gas chromatography–electroantennogram detection (GC-EAD) was used to identify which compounds induced beetle olfactory responses. These were identified by gas chromatography–mass spectrometry (GC-MS). Behavioural responses of bark beetles were tested using a Y-tube olfactometer.

Results

Several EAD peaks were recorded, of which trans-4-thujanol was consistently active and could be identified chemically unequivocally. The antennae of I. typographus females responded to a lower dosage of the compound than males, but both sexes were repelled by it. The bark of 10-year-old spruce trees contained 3 times more trans-4-thujanol than that of 35- 40-year-old trees, 27 times more than that of 70- to 80-year-old trees, and 200 times more than that of 120-year-old trees.

Conclusions

Trans-4-thujanol is a bioactive compound in Norway spruce bark that varies in amount with tree age and affects spruce bark beetle behaviour under laboratory conditions. It is suggested that trans-4-thujanol might play a role in both spruce tree defence and tree choice by beetles.

1 Introduction

The bark beetle, Ips typographus (L., 1758) (Coleoptera, Scolytidae) is one of the most significant pests of Norway spruce [Picea abies (L.) Karst.]; it can destroy large numbers of mature trees and cause substantial economic losses (e.g. Mezei et al. 2014; Wermelinger 2004). The colonisation of spruce trees is initiated by pioneer I. typographus males. After excavation of nuptial chambers, they release an aggregation pheromone that attracts both female and male conspecifics. This triggers a mass attack on a selected tree (Byers 1989; 2004). Small populations of I. typographus colonising weakened host trees can increase dramatically to epidemic levels, usually after windstorms or extended periods of drought, destroying large areas of Norway spruce forest (Långström et al. 2009; Wermelinger 2004).

Volatiles released by the host plant play an important role when pioneer bark beetles search for a host tree (Andersson 2012; Erbilgin et al. 2007). Ips typographus can perceive at least 18 compounds emitted by its host plant (Kalinová et al. 2014). Electrophysiological recordings from individual olfactory sensilla on I. typographus antennae indicate that approximately 42 % of olfactory receptor neuron cells are specialised for pheromone perception, 35 % for perceiving host-plant compounds, and at least 23 % for detecting non-host-plant compounds (Andersson et al. 2009). Behavioural assays have demonstrated that several non-host compounds can inhibit I. typographus beetle attraction to the pheromone (Zhang et al. 1999; Zhang and Schlyter 2003). Hence, volatiles released both by host- and non-host-plants are of utmost importance when beetles search for a host tree (Zhang and Schlyter 2004).

Many organic plant-released compounds act either as pheromone synergists or antagonists to insect herbivores (e.g. Byers 1989; 2004; Landolt and Phillips 1997). For I. typographus for example, α-pinene—the predominant monoterpene in Norway spruce—enhances the attractiveness of the aggregation pheromone (Erbilgin et al. 2007; Jakuš and Blaženec 2003). Conversely, 1,8-cineole acts as an antagonist inhibiting attraction to the aggregation pheromone (Andersson et al. 2010). A field survey indicated that trees that were attacked but not colonised during an outbreak exhibited a greater content of 1,8-cineole than successfully colonised trees (Schiebe et al. 2012), suggesting the involvement of this compound in tree resistance. The question arises whether 1,8-cineole is the only spruce volatile compound potentially involved in resistance mechanisms or whether other similarly acting compounds are produced and released by spruce trees.

Some volatile plant secondary metabolites sensed by bark beetles remain unknown, as proved by electrophysiological analysis of beetle receptor neurons in olfactory sensilla: it is still not known what substances approximately 24 % of receptor cells respond to (Andersson et al. 2009). Ips typographus preferentially infest old (over 100 years) and large (Becker and Schröter 2000; Zolubas et al. 2009) spruce trees. To discriminate trees suitable for colonization, bark beetles rely on several stimuli, probably including host volatile compounds. Hence, comparative analysis of volatiles emitted by old and young spruce trees could lead to identification and use of some of these compounds in bark beetle control programmes. The objective of the present study was to find spruce tree-produced volatiles to which I. typographus beetles respond and that vary in production as the plant ages. Knowledge of spruce tree volatile compounds that affect the behaviour of I. typographus beetles, in particular those that act as antagonists of attraction, may have important applications, e.g., for the use of olfactory stimuli in integrated forest protection against bark beetles.

2 Materials and Methods

2.1 Insects

The I. typographus adults used for analysis were obtained from pheromone (commercial attractant Ipsodor, Chemipan, Warsaw, Poland) multi-funnel traps hung in forest fresh clear-cuttings or were collected under the bark of naturally attacked Norway spruce trap-trees from April to September in 2011 and 2012, in Užtrakis forest (54°35΄N; 24°57΄E), Trakai district, Lithuania. The beetles were selected randomly for the bioassay. Live beetles (for antennal recordings and behavioural assays) were maintained at room temperature (ca. 23 °C) in a glass container (28 L) with spruce bark chips. The sexes were separated based on differences in the density of bristles on the pronotum and size differences of the frontal tubercle and third elytral spine (Schlyter and Cederholm 1981). To ensure correct sexing, the beetles were dissected after the experiments.

2.2 Bark sample collection

Norway spruce bark was collected in Sužionys forest (54°58΄–55°02΄N; 25°27΄–25°30΄E) in Vilnius district, Lithuania. In August–September 2010 and in April 2011, trees selected randomly from each of the four populations of 10- (N = 9), 35- to 40- (N = 5), 70- to 80- (N = 7) and 120-year-old trees (N = 5) were sampled for bark. The bark (1.5 × 12 cm, about 6 g) was taken from the southern side of the trunk at a height of 50–100 cm from the 10-year-old trees and a height of 180 cm from the 35- to 40-, 70- to 80-, and 120-year-old trees, i.e. in the lower one-third of the trunk. Bark samples were transported in small hermetically sealed plastic bags and kept in the laboratory at −18 °C until extraction.

2.3 Bark sample extraction

Norway spruce bark extracts were prepared by hydrodistillation: 5 g bark was cut into small pieces, soaked in water for 1 h, and boiled in a Clevenger type apparatus for 2 h (Eur. Ph. 1996). Volatiles were collected into 3 mL of a mixture of hexane and diethyl ether (2:1). The extract was dried over MgSO₄, filtered and stored in a freezer at −18 °C until gas chromatography–electroantennogram detection (GC-EAD) and gas chromatography–mass spectrometry (GC-MS) analyses.

2.4 Gas chromatography–electroantennogram detection

For the GC-EAD analysis, bark extract from 40-year-old trees was used because trees become attractive to bark beetles starting from 40 years of age (see CFIA 2012). Bark extract (1 μL) was injected splitlessly into a Clarus 500 gas chromatograph (PerkinElmer, Waltham, MA) equipped with a DB-Wax capillary column (30 m; 0.25 mm i.d., 0.25 μm film-thickness, Agilent Technologies, Santa Clara, CA) and a 1:1 effluent splitter that allowed a simultaneous flame ionization detection (FID) and EAD of the separated volatile compounds. Hydrogen was used as carrier gas (2.5 mL/min). The injector temperature as well as that of the detector was set at 240 °C. The column temperature was maintained at 40 °C for 1 min, after which it was raised to 200 °C at a rate of 5 °C/min, then increased to 240 °C at a rate of 10 °C/min, and maintained isothermally for 11 min.

The outlet for the EAD was held in a humidified air-stream flowing at 0.5 m/s over an I. typographus antennal preparation (Zhang et al. 2000). A glass capillary indifferent electrode filled with 0.9 % NaCl saline (Ilsanta, Vilnius, Lithuania) and grounded via a silver wire was inserted into the severed beetle head with antennae. A recording electrode connected to a high-impedance DC amplifier with automatic baseline drift compensation was placed in contact with the distal end of an antenna. The signal was stored and analysed on a PC equipped with an IDAC-card using the program GcEad V. 4.4 (Synthech, NL 1998). A total of 12 antennae from different males were used for the GC-EAD analysis.

2.5 EAG dose-response

Using the same electrophysiological recording setup and the same antennal preparation technique, electroantennogram (EAG) dose–responses of I. typographus males and females to synthetic trans-4-thujanol were recorded. Stimuli prepared by diluting the EAD-active compound in 10 μL hexane were applied to a piece of filter paper (5 × 45 mm) in a Pasteur pipette; four concentrations were tested in ascending order (0.1, 1, 10, then 10² μg). A solvent blank (10 μL hexane after evaporation) was tested as a control stimulus before the lowest and after the highest dosage of the test compound. We recorded the peak voltage amplitude during the puff delivery of each stimulus as an antennal response. Each stimulation was followed by a minimum of a 2-min purge period of filtered air to ensure recovery of antennal receptors. The EAG responses to EAD-active compound doses (R) were calculated according to the formula: R = R _A − (R _C1 + R _C2)/2, where R _A was the recorded EAG response to the EAD-active compound, and R _C1 and R _C2 were EAG responses to the first and the second control stimuli.

2.6 GC-MS analysis

Bark extracts were analysed using a Varian 3400 GC (Varian INC, Palo Alto, CA) connected to a Finnigan SSQ 7000 MS (Thermo-Finnigan, San Jose, CA) with a DB-WAX fused silica capillary column (30 m; 0.25 mm i.d., 0.25 μm film-thickness, J&W Scientific, Folsom, CA) and Shimadzu GC-MS-QP2010 Plus (Shimadzu, Tokyo, Japan) with a Rxi-1 ms fused silica capillary column (30 m; 0.25 mm i.d., 0.25 μm film-thickness, Restek, Bellefonte, PA). The temperature was programmed as follows: the initial temperature of 40 °C was kept for 1 min, after which it was increased to 200 °C at a rate of 5 °C/min, then raised to 230 °C at a rate of 10 °C/min, and maintained constant at 230 °C for 23 min. The injector temperature was 230 °C. Helium was used as carrier gas (inlet pressure 70 kPa). Electron ionisation spectra were acquired at an electron energy of 70 eV, and the ion-trap source temperature was 150 °C. The sample (1 μL) was introduced to the injector manually. The compounds were identified by comparing retention indexes (RIs) and mass spectra with NIST (National Institute of Standards and Technology) reference libraries and with an authenticated standard (below).

2.7 Isomer identification

The synthetic standard of 4-thujanol (2-methyl-5-(1-methylethyl)bicyclo[3.1.0]hexan-2-ol) (CAS Nr. 546-79-2) was obtained from Fluka (Seelze, Germany). Shimadzu GC-MS-QP2010 Plus (Shimadzu) was used for GC–MS analyses of the 4-thujanol standard on a non-polar (RI 1052, Rxi-1 ms) and a polar (RI 1465, DB-Wax) column to identify the isomer. The temperature was programmed as described above. The structure of the compound was confirmed by nuclear magnetic spectroscopy (NMR). The NMR data were obtained using Bruker DRX-400 NMR instrument equipped with a 5 mm inverse broadband probe head. The 2D NMR experiments (COSY, NOESY, HMQC) were measured by running standard Bruker pulse sequences at room temperature.

2.8 Y-tube olfactometer bioassays

Olfactory responses of I. typographus adults in pedestrian bioassays were tested using a Y-tube olfactometer modified from Schlyter and Löfqvist (1986). The Y-tube olfactometer consisted of the following parts: a release chamber, a Y-tube (internal diameter 0.7 cm; length of arms 4 cm), a beetle trap (10 mL vials on each arm of the olfactometer), and a stimulus-holding tube containing a piece of filter paper (5 × 45 mm) with stimuli (also on each arm of the olfactometer). Filtered, humidified air (1.6 mL/s) carried odours from the two stimulus-holding tubes to each arm of the Y-tube.

Solutions of 200 mg/mL and 20 mg/mL of synthetic trans-4-thujanol in hexane (for GC ≥ 99.0 %, Fluka) were prepared and 10 μL sample was then applied to filter paper squares, giving doses of 20 μg and 200 μg, respectively. The solvent was allowed to evaporate for 20 s before the papers were placed inside the sample tubes. Ten microlitres of hexane after evaporation was used as a control.

An hour before the test, the sexed beetles were placed in Petri dishes. About 35 (range 30–40) beetles of each sex were used in each test. A dose of 20 or 200 μg trans-4-thujanol was tested using three and four replicates on females, four and six replicates on males, respectively. Three replicates were used for control.

The same beetles were tested once a day for 2 days and each test lasted 1 h. At the end of the test, beetles in different parts of the apparatus were counted. The individuals collected in a trap were recorded as responding to either the test odour or the control depending on the arm of olfactometer chosen. After each test, approximately 20 % of the beetles remained in the release chamber or in the first part of the Y-tube, having not responded to either of the stimuli. These beetles were excluded from data analysis. Between trials, we replaced Y-tubes with clean ones and swapped bait treatments to opposite arms to eliminate directional bias.

2.9 Statistical analysis

Statistical analysis was performed using Statistica 6.0 software (StatSoft, Tulsa, OK). The Wilcoxon paired signed rank test was used to compare antennal responses to EAD-active compound dilutions to the average of the contiguous control responses. Detection thresholds were determined as the lowest concentration at which compounds elicited significantly larger responses than those of the control. EAG amplitudes of beetle antennae of both sexes were compared using a Mann-Whitney U test.

A G-test for goodness-of-fit was used to identify significant preferences for one olfactometer branch in the Y-tube tests (Sokal and Rohlf 1995). We set the α level at 0.05 for all tests.

The absolute amount of trans-4-thujanol was calculated relative to the internal standard (tetradecane) and expressed as an equivalent (μg/g dry wt). The relative amount of trans-4-thujanol was calculated as the ratio of the area of its GC peak to the sum of all areas of the 13 monoterpene hydrocarbons in a defined GC fraction consisting of compounds eluting at RI from 998 to 1275 (see Table 1), and expressed as percentage.

Table 1 Amount of terpenes (%, mean ± standard error) in bark of Norway spruce (Picea abies) of different age

The Spearman nonparametric correlation (R _S) was used to determine the relationship between the age of Norway spruce trees and either the absolute or relative amount of trans-4-thujanol produced by the trees.

3 Results

3.1 GC-EAD recordings and GC-MS analysis

GC-EAD results demonstrated that extracts of spruce bark contained compounds that elicited antennal responses from bark beetles. One EAD-active compound (recorded in 10 of 12 runs, labelled 4 in Fig. 1) showed a mass spectrum very similar to that of trans-4-thujanol. The elution data of the natural product from polar and non-polar capillary columns closely matched those of the synthetic standard (Fig. 2). In conclusion, the EAD active compound present in Norway spruce bark extract was identified as trans-4-thujanol. The identity of five other EAD-active compounds remains tentative and additional experiments are ongoing.

Fig. 1

Flame-ionization (FID) and electroantennogram detector (EAD) recordings of antennal responses of Ips typographus males to Norway spruce bark extract. 1 Internal standard (tetradecane); 2 α-longipinene; 3 cyclosativene; 4 trans-4-thujanol; 5 ylangene; 6 copaene; 7, 8 unidentified

Fig. 2

Parts of chromatograms of Norway spruce bark extract (c) and trans-4-thujanol standard (d) on a non-polar column Rxi-1 ms, and b polar column DB-Wax. 1 Limonene; 2 γ-terpinene; 3 trans-4-thujanol; 4 terpinolene; 5 cis-4-thujanol; 6 α-longipinene; 7 cyclosativene; 8 ylangene; 9 copaene; 10, 11 unidentified

3.2 GC–MS and NMR spectroscopy analyses of standards

GC–MS analyses of the 4-thujanol standard based on its GC retention index on a non-polar (RI 1052, Rxi-1 ms) and a polar (RI 1465, DB-Wax) column showed trans-4-thujanol isomer (trans- for Me vs. IPP) (after Adams 2012; Umano et al. 2002) (Fig. 2).

The structure of the compound was confirmed by NMR spectroscopy. The one and two-dimensional ¹H and ¹³C NMR spectra proved the trans-structure unambiguously. In the ¹H-¹H 2D NOESY experiment, strong cross peaks appeared between the 10-methyl protons and the proton in position 1 and one of the methylene protons in position 6 (circled in the NOESY spectrum in Fig. 3a) indicating their steric proximity (arrows in Fig. 3b). The distance between the methyl protons and the corresponding methylene proton in position 6 (circles in Fig. 3c) in the cis- isomer is much larger and out of the range of the distance (ca. 4 Å) for a NOE observation.

Fig. 3

a ¹H-¹H 2D NOESY spectrum of trans-4-thujanol. b, c Geometry optimized structures for trans- (b) and cis- (c) 4-thujanol

3.3 EAG dose-response

EAG analysis of synthetic trans-4-thujanol confirmed the consistent response of bark beetle antennae. The EAG dose-responses to trans-4-thujanol indicated that the elicitation in bark beetles was not strong; the mean antennal response of male beetles varied from 0.028 ± 0.003 mV at the lowest dose (0.1 μg) to 0.078 ± 0.006 mV at the highest dose (100 μg). Meanwhile, the mean antennal response of females varied from 0.05 ± 0.003 mV to 0.062 ± 0.005 mV, respectively. A statistically significant difference in responses to 0.1 μg trans-4-thujanol was recorded between male and female beetles (Fig. 4), with males responding more weakly than females (Mann-Whitney U test; U = 52, P = 0.03).

Fig. 4

Electroantennogram (EAG; mean amplitude ± SE) dose–responses of I. typographus antennae to trans-4-thujanol. Asterisk indicates a significant difference between EAG responses of males and females (Mann-Whitney U test; P < 0.05)

The threshold dose of trans-4-thujanol that elicited a significantly stronger antennal response than the control was 1 μg (Wilcoxon test; Z = 2.95, P = 0.003) for males, and 0.1 μg (Z = 2.20, P = 0.028) for females.

3.4 Y-tube olfactometer bioassays

The control bioassay during which the same control stimulus (10 μL hexane after evaporation) was delivered to both branches of the Y-tube olfactometer showed that both females and males selected either branches of the olfactometer equally (males: G = 0.89, P > 0.05; females: G = 0.61, P > 0.05). When bark beetles were allowed to choose between 20 μg trans-4-thujanol and the control, neither sex demonstrated a preference (males: G = 0.22, P > 0.05; females: G = 0.82, P > 0.05). However, a ten-fold increase in the dose of trans-4-thujanol caused a significant preference of control versus monoterpene sample in both male (G = 4.6, P = 0.032) and female (G = 11.6, P < 0.001) bark beetles (Fig. 5). In conclusion, trans-4-thujanol at a dose of 200 μg acted as repellent to the bark beetles tested.

Fig. 5

Behavioural response of I. typographus beetles in paired choice test using a Y-tube olfactometer. T Choice of trans-4-thujanol (doses 20 μg and 200 μg), C choice of control (10 μL evaporated hexane). The difference in response was calculated using G-test; ns no significant difference

3.5 Trans-4-thujanol in host plant

The amount of trans-4-thujanol in spruce tree bark extracts was tree age dependent. On average, the amount extracted from 10-year-old spruce trees (18.1 ± 2.4 μg/g) was 3 times higher than in 35- to 40-year-old trees (5.6 ± 1.4 μg/g), 27 times higher than in 70- to 80-year-old trees (0.7 ± 0.3 μg/g), and 200 times higher than in 120-year-old trees (0.09 ± 0.05 μg/g). Both absolute and relative amounts of trans-4-thujanol in the bark of spruce trees negatively correlated to tree age (absolute amount: R _S  = −0.89, P < 0.001; relative amount: R _S  = −0.72, P < 0.001) (Fig. 6).

Fig. 6

Relationship between Norway spruce age and a the concentration (μg/g dry weight of bark) as well as b the relative amount (%, in relation to 13 monoterpenes) of trans-4-thujanol

4 Discussion

The electrophysiological response data of bark beetle antennae to volatiles produced by the host plant revealed the ability of I. typographus to perceive compounds present in spruce bark. Using the GC-MS method, one such compound was identified as trans-4-thujanol. Although this compound has been detected in the bark of Norway spruce (Schiebe 2012) and in volatiles collected from I. typographus males boring in spruce (Francke et al. 1995), no functional evidence of the compound has been reported. Some data on I. typographus EAG-active compounds, including trans-4-thujanol, have been presented previously (Blažytė-Čereškienė et al. 2009; Schiebe 2012), but here we report for the first time a behavioural effect of this compound, and the correlation between trans-4-thujanol amount and tree age.

The mere fact that bark beetles react to trans-4-thujanol at both the receptor and behavioural levels indicates the potential importance of this compound. Despite trans-4-thujanol being repellent for bark beetles under laboratory conditions, it remains to be investigated whether it is active in the field. We assume that this compound belongs to the group of already known substances decreasing attraction of I. typographus adults, including 1,8-cineole and (−)-limonene both produced by spruce trees (Andersson et al. 2010; Schiebe et al. 2012), verbenone released by microorganisms in the galleries of bark beetles (review in Byers 2004) and green leaf volatiles emitted by many non-host trees (Zhang et al. 1999), as well as ipsenol and ipsdienol produced by I. typographus males to avoid competition in the later phases of tree attack (Birgersson et al. 1988).

Our results raise a question about the actual emission rates of trans-4-thujanol from spruce tree bark in nature. To address this, solid phase microextraction (SPME) or another technique for volatile collection would help determine the concentration of trans-4-thujanol in the vicinity of a trunk. It thus remains to be investigated whether this concentration of trans-4-thujanol is sufficient to induce behavioural reactions and to affect spruce tree selection by beetles.

Interestingly, the EAG dose-response recordings revealed some sexual differences in sensitivity: at low dosages, trans-4-thujanol induced higher EAG amplitudes in the antennae of I. typographus females than in those of males (Fig. 4). This may indicate that female antennae contain more receptor cells that respond to this compound, supporting the data obtained by single-cell activity recordings that showed that females contain a higher proportion of receptor neurones tuned for compounds of host-plant origin than do males (Andersson et al. 2009). I. typographus attacks many more spruce trees than those that become successfully colonised and subsequently killed (e.g. Schiebe et al. 2012). Spruce tree attack is initiated by pioneer males selecting the tree, excavating a short nuptial chamber and starting pheromone release. In the case of unsuccessful attack, the pioneer beetles either drowned in resin or had left their entrance holes after 3 days when no further beetles joined their attack (Schiebe et al. 2012). It remains unclear why conspecifics sometimes do not join the attack. During the early attack phase nearly 60 % of arriving females either fly away or drop down from the tree already settled by pioneer males (Paynter et al. 1990). One may assume that input from females joining pioneer males is important in the success of an attack, especially keeping in mind that females join the attack shortly after the pioneer males start producing and releasing aggregation pheromone, i.e. during the initial phase of the attack (from few hours to1 day, Schlyter et al. 1987). In addition, females have higher tunnelling activity than males (Faccoli and Schlyter 2007), and are more abundant in colonised trees than males (Wermelinger 2004). Tree resistance decreases as phloem infestation increases (Lieutier 2004), and females contribute significantly to this process by boring egg-galleries (Faccoli and Schlyter 2007). If females do not make the galleries, the tree might survive the pioneers’ attack. The involvement of females in the exhaustion of tree defences is under debate (Faccoli and Schlyter 2007; Lieutier et al. 2009). However, it could be hypothesized that, if the females are more sensitive to trans-4-thujanol than males, they could reject some trees already selected by pioneer males, thus decreasing the chance of a successful attack. Double choice may increase the probability of colonising only the most suitable trees.

As suitable spruce choice by females determines offspring survival, selection of a correct location for egg-laying is critical for progeny. We assume that the repellent properties of trans-4-thujanol might be involved in the process. Moreover, the compound is not specific to the spruce tree, as trans-4-thujanol is present in many plant species and especially abundant in those of medicinal importance (e.g. Groendahl et al. 2008; Novak et al. 2000), and which are well known for their fungicidal properties. For instance, essential oil of marjoram contains trans-4-thujanol as one of the main components (Novak et al. 2000), and the oil acts as a fungicide (Sharma et al. 2011). We assume that trans-4-thujanol may have an antifungal property, like some Norway spruce monoterpenes, which can inhibit growth of the blue stain fungus, Ceratocystis polonica (Siem) C. Moreau (Novak et al. 2014). As fungi within galleries excavated by bark beetles are important for I. typographus larval feeding (Lieutier et al. 2009), the presence of a certain concentration of trans-4-thujanol might be unfavourable for bark beetle broods. Thus, the females’ higher sensitivity to the repellent compound trans-4-thujanol might ensure selection of the most suitable locations for egg-laying.

It is well known that ageing spruce trees become predisposed to bark beetle infestation (e.g. Grodzki et al. 2014; Netherer and Nopp-Mayr 2005). When trees are less than 40 years old, spruce stands sustain very little damage (CFIA 2012), but tree mortality caused by bark beetle attack increases when stands are older than 50–60 years (Grodzki et al. 2006). Our results indicate that the concentration of trans-4-thujanol decreases in ageing spruce trees, and does so alongside the concentration of other closely related secondary metabolites, i.e. monoterpenes (Table 1). Thus, the emission of trans-4-thujanol might allow I. typographus to discriminate suitable and/or older trees. To compare our results with those obtained by other authors on bark chemistry dynamics in ageing spruce trees is hardly possible becuase, to our knowledge, no similar data have been published other than the well documented dynamics of ageing seedlings (Kännaste et al. 2013).

In summary, our results show that trans-4-thujanol produced by spruce trees triggers a behavioural response in I. typographus. Laboratory bioassay revealed the repellent properties of the compound. The data presented in this paper raises questions regarding whether trans-4-thujanol affects bark beetle behaviour in the field, which could be useful in integrated forest protection against bark beetles, and whether the compound may reflect substrate quality for brood development. Further experiments would be required to test these hypotheses.