Pollination success of Fraxinus excelsior L. in the context of ash dieback
Annals of Forest Science volume 80, Article number: 22 (2023)
Paternity analyses show that effective pollination of ash (Fraxinus excelsior L.) in a seed orchard and a floodplain forest affected by ash dieback is more likely to be facilitated by healthier males. Thereby, natural selection can have a positive effect on the health of future generations.
Ongoing ash dieback and increasing fragmentation of ash populations may result in reduced pollen flow, which can reduce pollination success of future generations of ash trees. Therefore, it is essential to further improve our understanding of gene flow patterns, especially with respect to ash dieback.
In this study, paternity analyses were conducted in a seed orchard and a floodplain forest in Germany in 2018 to explain the relationship between pollination success and the health status of ash trees and distances of effective pollen transport.
Cambium samples (i.e., from twigs and stumps) were collected from 251 ash trees (putative father and mother trees) for genotyping, and the health status of each tree was documented using a scoring system to evaluate vitality. Additionally, seeds were harvested from 12 mother trees per site. Genetic analyses using nuclear microsatellites were performed to determine paternal trees. Paternities were assigned based on the likelihood model implemented in the Cervus 3.0.7 software.
Our results showed that the average pollination distance was 76 m in the seed orchard and 166 m in the floodplain forest. In general, pollination success decreased substantially with increasing distance to the mother tree. Despite the dense tree cover in the floodplain forest, pollen were transported over long distances (greater than 550 m), suggesting that non-local sources also play a role in pollination. This is supported by the foreign pollen input identified in the seed orchard (66.5%). Self-pollination was detected only to a very small extent, and thus had no major influence on reproduction. In addition, both healthy and slightly diseased father trees showed similar mating success. However, this was not the case for the severely diseased ash trees (more than 50% of crown damage) because only a few offspring could be assigned to them. Nevertheless, in contrast to the floodplain forest, there was no significant correlation between damage classes and pollination success in the seed orchard.
Long-distance pollen transport contributes to the connectivity of ash trees in the landscape. Additionally, both healthy and slightly diseased fathers have a greater contribution to pollination, thus potentially improving the health of the next generation of ash trees. Moreover, gene flow between stepping stone populations is necessary to ensure the positive impact on the genetic diversity of ash populations in the future.
In recent decades, ash dieback, caused by the fungus Hymenoscyphus fraxineus (T. Kowalski) Baral, Queloz, and Hosoya (Baral et al. 2014) (Syno.: Hymenoscyphus pseudoalbidus (Queloz et al. 2011) and its asexual stage Chalara fraxinea (Kowalski 2006), has acutely threatened ash populations in Europe (Metzler et al. 2012; McKinney et al. 2014; Kosawang et al. 2018) and its silvicultural future (Enderle 2019). Common ash (Fraxinus excelsior L.) is considered a promising tree species under climate change conditions (Kölling 2007; Enderle et al. 2017; LWF 2019; Müller-Kroehling and Schmidt 2019) and presents itself as a versatile and valuable species with high ecological and economical importance (Enderle et al. 2017; Hultberga et al. 2020). However, high mortality rates have already led to a sharp decline in many local ash populations (Lygis et al. 2014; Marçais et al. 2017; Pliūra et al. 2017; Semizer-Cuming et al. 2021). Simulations indicated that up to 75% of ash trees in mixed stands in Europe are expected to die over the next 30 years (Coker et al. 2019). Other studies even suggest that only approx. 1–5% of ash trees are not susceptible and show little or no symptoms related to ash dieback (McKinney et al. 2014; Rigling et al. 2016; Enderle 2019). In this regard, individuals with very low levels of disease symptoms and less than 30% leaf loss are considered partially resistant (i.e., developing less symptoms) and might be suitable for genetic conservation or breeding programs (Lenz et al. 2012; McKinney et al. 2014). Disease development at stand level will therefore depend on the ability of less susceptible genotypes to spread their genes via pollen and seeds (Lobo et al. 2015; Semizer-Cuming et al. 2019, 2021). Partial resistance to ash dieback was not found to be population or provenance based but rather individual based (McKinney et al. 2011, 2014; Enderle et al. 2017; Semizer-Cuming et al. 2019). Therefore, it is essential that genetic connectivity is maintained, even in fragmented landscapes, and that genetic diversity is sufficiently high for the reproduction of trees for future healthy ash populations. To assess tree population responses and the consequences of ecological and biological threats, it is essential to understand pollen dispersal patterns.
Effective gene flow between partially resistant adult trees is necessary to establish healthier next generations of ash trees (Semizer-Cuming et al. 2017, 2019; Fussi 2020). However, ongoing ash mortality and the increasing fragmentation of ash populations lead to a decrease in pollen flow (McKinney et al. 2014; Semizer-Cuming et al. 2017), which can further limit the genetic diversity of future generations (Fussi et al. 2014). If partially resistant ash trees are highly underrepresented, less healthy genotypes will also succeed and produce offspring. This can lead to decreased natural selection under the prevailing environmental conditions (Eisen et al. 2022b). Reduced genetic diversity can negatively affect the adaptive potential of future generations of ash trees, whereby genetic variation is particularly important for adaptation to new pathogens due to the long generation time of forest trees (Fussi et al. 2014; McKinney et al. 2014). In addition, ash trees are trioecious, i.e., monoecious and dioecious individuals can occur simultaneously (Roloff 1997), which can increase the risk of self-pollination (McKinney et al. 2014; Semizer-Cuming et al. 2021).
Genetic analyses investigating the mating success of ash individuals in populations have been performed only in a few studies to date (Heuertz et al. 2003; Bacles and Ennos 2008; Thomasset et al. 2014; Semizer-Cuming et al. 2017). The relationship between reproductive success and the health status of ash trees in a seed orchard has only been assessed once (Semizer-Cuming et al. 2019): It was demonstrated that health status and reproductive success of ash trees in a seed plantation in Denmark were negatively correlated. Female ash trees severely affected by ash dieback produced a much lower seed quantity compared to healthy ones, whereas damaged males could still sire some offspring. In another study, Semizer-Cuming et al. (2017) examined the genetic connectivity of ash trees in an isolated forest area. Thereby, it was found that about 55–64% of the seeds and 75–98% of the seedlings descended from local ash trees, but 26–45% of the pollen were transported from outside the forest area. Moreover, a positive correlation was found between seed dispersal distance and wind speed, while there was none between pollen dispersal distance and wind speed. Research on distances of effective pollen transport varied widely among studies: Heuertz et al. (2003) conducted a study in a mixed deciduous forest in Romania and determined a distance of 70–140 m, whereas Bacles and Ennos (2008) estimated the distance with up to 2.9 km in a deforested Scottish landscape. Semizer-Cuming et al. (2021) found that 50% of pollen dispersal occurred within 140 m in a Danish forest, 5% of pollen dispersal occurred over a distance of 1.3 km, and 1% was transported farther than 3 km. Similar results were also obtained from aerobiological investigations on pollen transport and deposition using gravimetric pollen traps, which were conducted in two ash seed orchards in Germany (Eisen et al. 2022a). It was shown that 50% of the ash pollen in the air occurred within a distance of 200 m from the source, whereas only 10% of the total pollen catch reached a distance of 500 m.
In general, pollen dispersal is influenced not only by abiotic and biotic factors such as meteorological conditions, topography, and vegetation but also by tree height and crown size (Scheifinger et al. 2013; Puc 2012; Adams-Groom et al. 2017; Eisen et al. 2022a). Sork and Smouse (2006) also noted that less fragmented landscapes have more pollen input from outside and therefore adjacent pollen sources. The magnitude of influential factors suggests that it is important to improve our understanding of gene flow patterns in fragmented and non-fragmented ash populations further to predict accurate estimates of pollen dispersal (Semizer-Cuming et al. 2017).
Given this background, the objectives of this study are thus to explain the relationship between pollination success and the health status of ash trees and to test whether healthy fathers contribute more to the next generation, as well as to estimate pollen dispersal at sites affected by ash dieback. Based on the results, we discuss the implications of the observed relationship between the extent of damage caused by ash dieback and pollination success in relation to conservation and management of the species. This could be of particular interest for the establishment of future seed orchards where pollen emission from outside should not influence the production of healthy offspring in the orchards.
2 Material and methods
2.1 Study area
The seed orchard Schorndorf (48°46′ N, 9°25′ E, 420 m a.s.l.) is located in the valley Remstal near Schorndorf, Baden-Württemberg, Germany, and has an area of approx. 2.3 ha (Fig. 1a). The average annual temperature is 10.3 °C (DWD station Stuttgart Schnarrenberg, 1981–2010) and the average annual precipitation 855 mm (DWD station "Winterbach, Rems-Murr-Kr.", 1981–2010). The orchard is located on a NW exposed slope with an inclination of about 6 to 10° (LGRB 2021). It consists of grafts of selected so-called “plus trees,” which were selected for growth and stem quality prior to the outbreak of the disease and is characterized by a well-spaced plot design (7 m × 7 m). The plus trees originated from the South German hill and mountain area and the Alps and Alpine foothills in Baden-Wuerttemberg (Enderle et al. 2014). Initially, 68 clones (416 ash trees) were planted in November 1992 in 25 rows: 36 clones with 8 female or hermaphrodite ramets and 32 clones with 4 male ramets. Due to the negative effects of ash dieback, the majority of the trees (ca. 70%) died (Eisen et al. 2022a). In 2018, the seed orchard consisted of 123 mature ash trees with a maximum height of 17 m (Fig. 1b/Appendix Fig. 7). In the seed orchard, common ash is growing almost exclusively with few other tree species such as cherry and apple trees, wild service trees, or lime trees (especially along the sides). It is surrounded by meadows, which are mostly bordered by a mixed forest consisting mainly of beech, spruce, and pine. There are no other ash trees in the immediate surroundings (within a radius of about 1 km). Due to ash dieback, there is currently no commercial demand for ash seeds.
The floodplain forest is located at the Danube between the cities Neuburg and Ingolstadt, Bavaria, Germany (Fig. 1a). The selected area for our investigations is situated near the Bergheim barrage (48°44′ N, 11°16′ E, 375 m a.s.l.) and has an area of about 10 ha. The average annual temperature is 7.8 °C, and the average annual precipitation is 715 mm (1961–1990) (Schwab et al. 2018). The calcareous and nutrient-rich substrate of the site enables favorable growing conditions especially for common ash (Doben et al. 1996; Margraf 2004). Common ash is represented in the dense floodplain forest with a share of about 15% (Jochner-Oette et al. 2021). In the selected area, 50 ash trees and 78 tree stumps were sampled for our analyses (Fig. 1c/Appendix Fig. 8).
2.2 Sampling and vitality assessment
In October 2018, a lift truck was used to collect seeds for genotyping in the seed orchard and in the floodplain forest (12 mother trees selected per stand, Table 1) to quantify the proportion of pollination success of diseased and healthy father trees. All investigated ash trees (seed orchard: 123 ash trees and floodplain forest: 50 ash trees) were classified according to the scoring system of Lenz et al. (2012) for assessing the vitality of adult ash trees. Trees in categories 0 and 1 (up to max. 30% leaf loss) were classified as healthy, all other trees as diseased (categories 2 to 4) or dead (category 5). The dead trees were not included in the surveys. In Schorndorf, seeds were collected from nine different clones, representing six healthy and six diseased ash trees. From three clones, two ramets were selected for seed harvest (Table 1). A total of five healthy and seven diseased ash trees were selected in the floodplain forest. The number of analyzed and genotyped seeds per mother tree varied between 48 and 49 seeds in Schorndorf and between 70 and 76 seeds in the floodplain forest.
In spring 2019, twigs from all 123 mature ash trees (12 mother trees and 111 adult ash trees) were sampled in the seed orchard. In the floodplain forest, twigs or wood from 128 trees were sampled: 50 samples were derived from standing ash trees and 78 from ash stumps. The 78 ash trees had to be removed in January 2019 in order to ensure road safety. Since damage caused by ash dieback had already been observed before, they were classified as diseased. The logged trees were ash trees, which may have served as pollen donors since they were cut after the pollen season and may have contributed to pollination and seed development. The standing ash trees were sampled at a 25-m radius around the mother trees, while ash stumps were interspersed (Fig. 1c/Appendix Fig. 8). After sampling, the tissue was preserved at − 20 °C until further analyses.
Due to the identical genotypes of the clones in the seed orchard, in most cases, a single tree could not be identified as the father (except for the cases where there was only one potential father per clone). Therefore, all ramets of a clone that are possible pollen donors for a mother tree were defined as potential fathers. To exclude the trees that were not candidate fathers (i.e., females and/or trees with no flowering), the gender of the ash trees was determined on site (in the period 2018–2021), and the presence of flowers or flower buds and their phenological stage was recorded thoroughly with binoculars on 19 April 2018 (109th day of the year). Hermaphrodite mother trees were also considered as potential father trees. For the floodplain forest, no phenological data were available in 2018. The gender was determined for the ash trees alive; however, this was not possible for the ash stumps. The evaluated attributes for all ash trees can be found in the Appendix (Tables 2 and 3). The geographic position (UTM coordinates; 32 N) of each sampled ash tree was recorded using DGPS (Stonex S9 III, Stonex, Paderno Dugnano (MI), Italy) for both sites (Appendix Fig. 7/Fig. 8).
2.3 DNA extraction and genotyping of microsatellite markers
For the adult trees, cambium of the twigs and stumps was used for DNA extraction. For the seeds, DNA was extracted from their embryos. DNA extraction was performed using the cetyltrimethylammonium bromide [CTAB] method (Doyle and Doyle 1990). The DNA content of the samples was determined using a spectrophotometer (GeneQuant pro, Amersham Biosciences, CA, USA). After DNA extraction, polymerase chain reaction (PCR) was performed to examine 15 microsatellite loci in three different multiplexes (Appendix Table 4) (Brachet et al. 1999; Lefort et al. 1999; Gerard et al. 2006; Aggarwal et al. 2011; Bai et al. 2011; Noakes et al. 2014). However, reproducible results could only be obtained for eleven markers (M2-30; Fp18437; Femsatl 11; Femsatl 12bis; Femsatl 4; Fp14665; Fp21064; FRESTSSR308; FRESTSSR528; Femsatl 19; ASH2429). Femsatl12bis had too many null alleles in floodplain samples, while the same was the case for marker Fp14665 in the seed orchard. These markers were therefore only used for the seed orchard and floodplain, respectively. Subsequently, PCR products were separated by high-resolution capillary electrophoresis using the GeXP automated sequencer (Beckman Coulter, Inc., Fullerton, CA, USA) and analyzed by software-assisted allele scoring.
2.4 Data analysis
For clone identification, the population genetic software GenAlEx 6.0 (Peakall and Smouse 2012) was used. The multilocus genotype of all adult trees was compared and identical genotypes assigned to one clone in the seed orchard. Trees with nonidentical multilocus genotypes with any of the known clones were treated as an individual tree and labeled “0000.” This was the case when the rootstock had grown up to a new tree (two variants: variant 1: The rootstock has grown up after planting, so the ash trees are sexually mature and could be potential fathers; variant 2: Due to ash dieback, the trees have died, and the rootstocks have sprouted. These ash trees were still too young for flowering). Paternity analysis implemented in the Cervus 3.0.7 software package (Kalinowski et al. 2007) was applied to determine the father of each seed. In this context, the allocation is based on Delta (Δ) (Labuschagne et al. 2015). Thereby, the difference of the likelihood-odds ratio (LOD) score between the two most likely parents is examined to find the actual parents. The critical values of Δ were calculated at strict (95%) and relaxed (80%) confidence levels during the simulations (Labuschagne et al. 2015; Semizer-Cuming et al. 2019). For Schorndorf, 100,000 offspring and 70% of sampled potential fathers were simulated. For the alluvial forest, 100,000 offspring and 30% of sampled potential fathers were simulated. The minimum number of loci was set to eight. The error rate was kept at 0.01 (Semizer-Cuming et al. 2019). In addition, self-pollination was explicitly taken into account in the model.
Pollen distances were calculated from the UTM coordinates of the mother and father trees. In the case of the seed orchard, the distances to the mother trees were calculated individually for all potential fathers (ramets) of the same clone, assuming that each of them could be the potential father, since it was not possible to determine the distinct one. Then, we averaged the sum of the distances for each father tree (i.e., each clone). Thus, it was only possible to estimate the average pollination distances to the mother trees. In the floodplain forest, we were able to calculate the actual pollination distances between fathers and mothers, as all had unique genotypes. The distances were classified at intervals of 10 m. In addition, the relationship between the number of offspring per father tree and the degree of damage to the father trees were calculated. Since ramets of the same clone were showing varying vitality scores, the average vitality score over all ramets per clone was calculated for the seed orchard Schorndorf. In order to estimate the correlation between the damage class and the pollination success, a chi-squared test and exact Fisher test were applied. All analyses and visualizations were performed in RStudio (version 1.2.1335.0) or Microsoft Excel 2016.
3.1 Seed orchard
3.1.1 Paternity analysis: relationship between reproductive success and health status
In total, 123 ash trees could be classified into 37 different clones with distinct genotypes. The number of ramets per clone varied from one to eight, with an average of four ramets per clone in the orchard. Twelve of the trees were selected as mother trees. Of the remaining 111 ash trees, 56 were male, 36 were female, and 15 were hermaphrodites. The gender of four ash trees could not be determined accurately. Seventy-six trees had flowers or flowering buds. Thus, 49 trees (36 females and 13 males/hermaphrodites without flowers) were excluded as pollen donors (Appendix Table 2).
Out of the 580 examined offspring, 194 (33.5%) of them could be assigned to their potential fathers (Fig. 2a). Five single trees and 18 clones (54 ash trees) were detected as potential fathers, although for four clones only one tree could be the father in each case with certainty, due to the exclusion procedure (ash trees that were female and/or not flowering were excluded as fathers). Due to its hermaphroditism and the fact that paternity could be found for this clone, one mother tree was also included as a potential father tree. Nine ash trees had no pollination success; three of them have not reached their reproductive age. Regarding the other six ash trees, four were hermaphrodite, and two were male. It was striking that the two male ash trees were very strongly affected by ash dieback (vitality scores 3 and 4).
The highest pollination success was observed for the clone 1651 (three potential fathers; average vitality score of 2) with 35 offspring, whereas the lowest pollination success was one offspring associated with 13 trees. Dividing the number of offspring per clone by the number of ramets, the maximum number of offspring is 11.7, and the minimum is 0.3. In addition, the average vitality score over all ramets of a father clone was analyzed; out of the 18 father clones and five single trees, three single trees could be assigned to an average vitality score of 0, six clones and two single trees to an average vitality score of 1, and ten clones to an average vitality score of 2. Only one clone each that sired offspring could be assigned to the vitality classes of 3 and 4 (one offspring each). Overall, father trees in vitality class 2 produced the most offspring (127 offspring) (Fig. 2b). In vitality score classes 0 and 1, in which the ash trees are still considered healthy, the number of identified fathers who sired offspring was substantially below the value of class 2 (vitality score 0: 5 offspring and vitality score 1: 60 offspring). However, no significant correlation could be detected between the damage classes and pollination success (chi-square test: p = 0.999, Fisher test: p = 0.997).
Considering the 54 potential father trees as individual trees (regardless of the clone affiliation), 6, 24, 18, 4, and 2 ash trees could be assigned to vitality scores 0, 1, 2, 3, and 4, respectively. With an average of 85.6 offspring, most of the father trees were detected in vitality score class 2 in this case as well. However, the number of fathers that sired offspring in scoring classes 0 and 1 was in total higher (vitality score 0: 11.1 offspring and vitality score 1: 82.0 offspring) than the number of offspring in scoring class 2, with an average of 93.1 offspring. Vitality scores 3 and 4 could be assigned an average of 10.6 and 4.7 offspring from identified fathers, respectively (Fig. 2c). Since vitality classes 1 and 2 had more ramets per clone, it can be assumed that weakly damaged clones have a higher overall potential to produce offspring. However, no significant correlation could be found (chi-square test: p = 0.982, Fisher test: p = 0.904).
For the mothers, the average number of offspring that could be assigned to a father tree was 7.5 offspring for vitality score 0 (two mother trees), 23.5 offspring for vitality score 1 (four mother trees), and 13.7 offspring for vitality score 2 (six mother trees).
The analyses identified possible self-pollination for three offspring of the mother trees 1619_31 (n = 2) and 1898_ 14 (n = 1). In the clone 1619 (n = 8), only the ramet 1619_86 was observed to produce male flowers in addition to female flowers. Thus, due to the number of ramets, it can be assumed that there is a low probability (11.1%) of self-pollination of the parent tree 1619_31. Clone 1898 consists of two female and six male ash trees; thus, the probability of self-pollination is again very low, with a value of 12.5%. No self-pollination could be detected for the two hermaphrodite mothers.
3.1.2 Distances of effective pollen transport
The average distance of effective pollen transport of all identified potential father trees to the mother trees was 76.2 m in the seed orchard Schorndorf (Fig. 2d). The largest distance was estimated at 191.1 m and could be detected between the potential father tree 1630_90 and the mother tree 1627_1. The shortest distance was calculated between the potential father tree 1644_32 and the mother tree 1619_31 at 6.3 m.
In general, it was found that only 5.3% of the identified paternal trees with potential pollination success were located less than 20 m from the mother tree. A total of 23.9% of the potential father trees were located between 21 and 50 m from the mother tree, and 45.1% of the potential father trees were located within a distance of 51 to 100 m. For the distance between 101 and 150 m, potential pollination success was attributed to 22.2% of the father trees and for the distance between 151 and 191.1 m only to 3.5%.
For a better spatial understanding of effective pollen transport, pollination success was investigated with respect to the geographical location of the mother trees. The two mother trees, 1619_81 and 1898_14, located in different areas of the seed orchard were selected as a representative to describe the general observed pollen dispersal patterns.
For mother tree 1619_81 (vitality score 1), most of the offspring (31 out of 48 seeds) could be assigned to their potential fathers (17 different trees) (Table 1). The mother tree is located in the southwestern area of the orchard, and thus, many potential father trees are within reach (Fig. 3a). The results showed that clone 1629 (four ash trees, average vitality score 1) had the highest pollination success (14 offspring) with this mother tree, but with a distance between 54.6 and 111.9 m, it is not located in the immediate vicinity of the mother tree. At the same time, it was found that the father clones with only one offspring are partly very close to the mother tree (clone 1651 — shortest distance of 34.9 m and clone 1879_75 — distance of 10.5 m). Both clones were linked to an average vitality score of 2.
The mother tree 1898_14 (vitality score 2) had the fewest offspring assigned to it (5 out of 48 seeds). However, for the five offspring, 16 possible father trees must be considered with only a maximum of one to two offspring descended from one father clone. The reason for this could be the location of the mother tree at the northern border of the orchard (Fig. 3b), since 43 offspring could not be assigned to a father clone. The potential father trees identified are not located directly around the mother tree but are mostly distributed in the southern part of the orchard. The closest potential father tree (1630_51; vitality score 3) was located at a distance of 48.1 m from the mother tree, 51.6% of the potential father trees were located between 51 and 100 m from the mother tree, and 44.8% of the potential father trees were located between 101 and 130 m from the mother tree.
3.2 Floodplain forest
3.2.1 Paternity analysis: relationship between reproductive success and health status
In the floodplain forest, all sampled trees had unique genotypes. In total, 224 (26.14%) offspring out of 857 examined could be assigned to 73 fathers (Fig. 4 a, b). Forty-three tree stumps and 30 ash trees were detected as father trees. From the 30 living ash trees, 2, 10, 9, 8, and 1 individuals had a vitality score of 0, 1, 2, 3, and 4, respectively (Appendix Table 3). Five of the living ash trees were hermaphroditic; the other 25 were male. Accordingly, no pollination success was detected for 20 living ash trees (out of 50 initially investigated trees), with only six of them being male and one being hermaphrodite; the other 13 were female. Please note that the cambium samples were collected prior to gender determination. Of these seven ash trees (male and hermaphroditic) without pollination success, two were healthy, and five were diseased.
The highest pollination success was observed related to the father tree tree stump 19 with 24 offspring, followed by the father tree A_22 with 20 offspring and the father tree A_01 with 13 offspring. Twenty-five trees only sired one offspring each. The highest total number of offspring was found in diseased ash trees (vitality scores 2–4). Thus, a significant correlation between the damage classes and pollination success could be found in this case (chi-square test: p = 0.001). The tree stumps, which were regarded as diseased individuals (n = 43), had 113 offspring (50.5%), and the diseased ash trees (n = 18) had 47 offspring (21.0%). In vitality scoring classes 0 and 1, in which the ash trees are considered as healthy (n = 12), the number of offspring was 64 (Fig. 4c). In addition, the average number of offspring per number of ash trees in a vitality scoring class was analyzed; it can be recognized that with an average of 12 offspring, father trees of vitality score 0 could be assigned to the most offspring. The father trees of vitality scoring classes 1, 2, 3, and 4 (n = 1) had an average of four, three, and two offspring, respectively, and the current tree stumps had an average of three offspring (Fig. 4d).
For the mothers, the average number of offspring that could be assigned to a father tree was 24, 18.5, 19.8, and 13.5 offspring for vitality score 0 (n = 1), 1 (n = 4), 2 (n = 5), and 3 (n = 2), respectively. Additionally, the analyses detected self-pollination for one offspring of the mother tree M_12 (hermaphrodite; vitality score class 3).
3.2.2 Distances of effective pollen transport
In the floodplain forest, the average distance of effective pollen transport was 165.6 m (Fig. 4e). The longest distance was 551.6 m and could be observed between the tree stump 79 and the mother tree M_08. The shortest distance (excluding inbreeding) with 2.3 m was calculated between the father tree A_02 and the mother tree M_07.
Overall, 9.6% of the father trees with pollination success were located less than 20 m from the parent tree, and 27.6% were located between 21 and 100 m away. Thus, it can be assumed that the nearest father trees did not pollinate most of the seeds. However, 49.3% of the father trees with pollination success were located within 130 m of the mother tree. A total of 33.3% of pollination success was detected for the trees at more than 200-m distance. For the distance between 301 and 400 m and 401 to 500 m, pollination success could only be detected for 6.4% of the father trees each. For the distance of 501 to 600 m, the value decreased to only 1.3%.
Detailed spatial information was acquired for two selected mother trees, i.e., mother tree M_04 and M_08. For mother tree M_04 (vitality score 2), 25 seeds out of 73 were attributed to their fathers. This was the mother tree with the highest number of seeds where the respective fathers could be identified. Fourteen father trees sired 25 seeds (Table 1). The mother tree was centrally located at the study site in a clearing northeast of the lake and was only obscured by other trees to the east and south due to its location (Fig. 5a). Therefore, many of the father trees investigated were within reach to the mother tree. The findings indicated that this mother tree had the highest pollination success (six offspring) with the tree stumps 64 and 68. Both father trees were located on the southwest side of the lake and were not in close proximity, with the distances of 179.9 and 196.8 m, respectively. In contrast, the father ash trees, which were not more than 100 m away from the mother tree, could only be associated with one offspring. In other words, the nearest fathers were not the most successful fathers.
Compared to M_04, the mother tree M_08 (vitality score 3) had less offspring assigned to their fathers. Seven father trees could be detected for the eight offspring. The mother tree is located at the northern edge of the study area; therefore, all identified fathers were positioned in the southwest of the mother tree (Fig. 5b). The closest father (A_06) with the pollination success of two offspring was located 40.6 m away from the mother tree. All other more distant father trees had only one offspring. The parent trees, tree stump 61 and tree stump 79, were located the farthest from the mother tree, at a distance of 374.3 and 551.6 m, respectively.
3.3 Comparative summary of the two study sites
The comparison of the results obtained from the seed orchard and the floodplain forest showed that the proportion of all investigated healthy ash trees (vitality scores 0 and 1) differed, with the orchard having 49% healthy trees (Fig. 6a) and the floodplain forest having only 15% (Fig. 6c) or 38% if excluding the tree stumps (Fig. 6e). The results were similar in terms of the proportion of ash trees that could be identified as (potential) fathers (Fig. 6b, d). In this case, the proportion of healthy ash trees was 50% in the seed orchard and 17% in the floodplain forest. However, considering the number of offspring, the analyses showed that at both sites, healthy fathers (vitality scores 0 and 1) as well as those not severely affected by ash dieback (vitality score 2) had almost the same mating success. In contrast, highly diseased fathers (vitality scores 3 and 4), whose proportion in the seed orchard was 14% and in the floodplain forest 30% (excluding the tree stumps; Fig. 6f), could only be assigned to a few offspring. Nevertheless, effective gene flow also takes place between highly susceptible trees.
Although the seed orchard offers the potential to capture most potential father trees, the proportion of offspring whose father could be identified was not noticeably lower in the floodplain forest either (orchard: 33.5%; floodplain forest: 26.1%). Our results on genetic connectivity showed that the average distance between identified father and mother trees was 76 m in the seed plantation and 166 m in the floodplain forest. Pollination success generally decreased substantially with increasing distance to the mother tree. Despite the dense tree coverage in the floodplain forest, pollen were transported over long distances (greater than 550 m). Thus, it indicates that local sources were not the only ones playing a role in the pollination. This is supported by the 66.5% of foreign pollen input found in the seed orchard. Nevertheless, we were able to identify the differences in pollen dispersal patterns, especially in the seed orchard. For instance, for mother trees at the border of the plantation (1627_01, 1878_13, 1898_14, 1627_21, 1866_30, 1663_94), only an average of 22% of the offspring could be assigned to their potential fathers, whereas the proportion for mother trees close to the center (1663_24, 1619_31, 1879_37, 0000_62, 1664_71, 1619_81) was 44%. A similar pattern was also found in the floodplain forest, though it was not as pronounced (near the center: 30%; at the edge: 21%).
4.1 Relationship between reproductive success and health status
Overall, our results showed that the ash trees of vitality score 2 produced the most offspring on average, both in the seed orchard and in the floodplain forest (excluding tree stumps). But, taking into account the number of individuals, healthy trees with no or little damage (vitality scores 0 and 1) were not associated with substantially lower pollination success. However, it should be noted that in the case of the Schorndorf seed orchard, only the average vitality score class of the clones could be considered for our assessments in the paternity analyses. Due to the identical genotype of the ramets per clone, the software cannot detect a distinct father tree. As the ramets per clone can have varying vitality scores, the average vitality score has been used. Therefore, it is possible that healthy ash trees will produce more offspring than diseased trees in a clone or vice versa. In the floodplain forest, some of the father trees were only present as tree stumps due to the tree removal before sampling. Therefore, we cannot accurately estimate their vitality score but assume that they were damaged by the fungus to some extent (vitality score > 2). Thus, based on the results, both healthy and relatively healthy fathers have high pollination success. However, the effect observed by Gassner et al. (2019) that the highest ash pollen emissions occurred 1 to 2 years after the first symptoms of ash dieback noticed in a region was very weakly perceived in our study. Similar effects of excessive flowering with increasing tree damage were also noted in the context of the forest dieback in the 1980s (Gassner et al. 2019). Thus, it appears that Hymenoscyphus fraxineus infection could at least temporarily increase pollen emission. This can be explained by the fact that stress symptoms in affected trees could lead to increased flowering (Wada and Takeno 2010), which can be regarded as a compromise between reproduction and defensive reaction of the tree (Denancé et al. 2013). Since disease resistance can be partially inherited from parents to offspring (Kjær et al. 2012; Lobo et al. 2015), increased flowering of damaged trees may have a negative effect on the natural regeneration. Thus, Kjær et al. (2012) suggested that only about 1% of the ash trees have the potential to produce offspring with an expected crown damage of less than 10% under the current disease pressure. Since our study revealed low pollination success for the severely damaged father trees and we observed a poor flower development, specifically on severely damaged ash trees in previous studies (Eisen et al. 2022a), it can be assumed that severe damage is associated with decreased pollen production. Nevertheless, even severely damaged male ash trees were capable of producing offspring, which is in line with the findings of Semizer-Cuming et al. (2019; 2021). On the other hand, crown damage caused by ash dieback reduces individual reproductive success (Semizer-Cuming et al. 2021) and thus has a positive effect on natural selection. Therefore, next generations will probably develop a higher resistance to ash dieback. This emphasizes the importance of not removing healthy ash trees during thinning as well as of enriching the gene pool of existing forests through targeted planting of less susceptible ash trees.
Additionally, self-pollination does not seem to have a major effect on reproduction in ash. According to our results, only in a very small proportion of the offspring, self-pollination could be detected with high probability (one offspring in the floodplain forest). Moreover, the study by Saumitou-Laprade et al. (2018) showed that Fraxinus excelsior L. might be self-incompatible, preventing loss of genetic diversity, which is critical for the future of ash, especially in the light of ash dieback (Fussi 2020).
Concerning the mother trees, in the seed orchard, the majority of the offspring (52.6%) whose fathers could be identified were assigned to mothers of vitality class 1, and in the floodplain forest, the proportion was 56.3%. Since the total number of seeds produced per tree was not investigated at this point, it is not possible to make a statement about the relationship between health status and reproductive success of the mother trees. However, a couple of studies previously reported that female ash trees severely affected by ash dieback produced much lower amounts of seeds compared to healthy ones (Semizer-Cuming et al. 2019, 2021). Overall, our research indicates a relationship between reproductive success and health, with healthy and only slightly damaged male ash trees (vitality score ≤ 2) contributing more to pollination.
4.2 Estimation of pollen transport distances
Our results showed that ash trees in Schorndorf had the highest pollination success rate (70%) within a radius of 100 m. In the floodplain forest, over 50% of pollination success occurred within 140 m, whereas only about 8% of father trees had pollination success over distances of more than 400 m. The longest detected distance of pollination in the floodplain forest was more than 550 m, corresponding nearly to the maximum extent of the study area. Thus, our findings are very consistent with previous research; the study by Heuertz et al. (2003), which revealed that effective pollen transport occurred at distances ranging from 70 to 140 m, fits well with our results from Schorndorf. The highest amount of successful pollination occurred at a distance of 76 m between father and mother trees in the seed orchard. In contrast, Semizer-Cuming et al. (2021) reported that 50% of effective pollen transport occurs within 140 m. This observation was reflected in the results of efficient pollen transport in the floodplain forest. In both cases, a sub-area of a large contiguous mixed forest was investigated, where ash occurs with other forest tree species.
Despite the fact that 2018 was a masting year at the Schorndorf seed orchard (Eisen et al. 2022a), a 66.5% foreign pollen input was detected. Seed orchards are collections of breeding populations that have been carefully selected to produce high-quality reproductive material (Fussi et al. 2014). Therefore, pollen input from ash trees from outside the orchard can affect seed quality. A seed orchard should be distinguished above all by the fact that the selected plus trees within the orchard mutually fertilize each other, with preferably no foreign input. Although no ash trees were found in the immediate vicinity of the orchard, it is possible that ash pollen from the further surrounding area contributed to fertilization. This has already been demonstrated in previous studies on effective pollen transport, which detected distances between 1.3 and 2.9 km (Bacles and Ennos 2008; Semizer-Cuming et al. 2021). In the 300-ha landscape heavily deforested described by Bacles and Ennos (2008), pollen immigration represented also between 43 and 68% of effective pollination (depending on the assignment method). Furthermore, Semizer-Cuming et al. (2017) found that 26–45% of pollen were transported from outside when they studied the genetic connectivity of ash trees in an isolated forest site in a fragmented landscape (2 ha). The cause of not local or even long-distance pollen transport could be wind related, which, as already demonstrated in other studies, is the primary factor responsible for the dispersal of pollen (Laaidi 2001; Eisen et al. 2022a). According to Puc (2012), larger effective pollen transport distances are linked to stronger winds. Semizer-Cuming et al. (2017) found a positive correlation between prevailing wind direction and the mean direction of pollen dispersal in ash. In addition, factors such as tree height and canopy width also influence pollen dispersal (Adams-Groom et al. 2017). Thus, pollination within the orchard may have suffered from many dead trees due to ash dieback. Thus, spacing between trees increased, possibly leading to an increasingly mixed pollen cloud and higher pollen income from outside to pollinate the flowers. This also corresponds well with our pollen dispersal patterns: In the case of mother trees in marginal positions, a greater pollen input from outside was observed, especially in the orchard. Therefore, seeds should be harvested preferably from central trees having a higher probability that both parents originate from plus trees within the orchards. In Germany, the Forest Reproduction Act (FOVG 2002; BGBl. I.S. 1658) recommends that seed orchards maintain a distance of 400 m from phenotypically weak stands of the same species or a species that can be crossed with it (gGA 2019). Thus, one measure to increase pollination within the orchard is to ensure that the distance between foreign ash trees and the seed orchard is substantially greater than 400 m. In addition, protecting seed orchards primarily with conifers such as spruce planted at the borders may aid in reducing pollen input from the outside and avoiding cross-pollination. Deciduous trees or shrubs might also reduce pollen input but are more permeable because leaf formation often begins after flowering of ash or are low in height (in case of shrubs). Another fact is the synchronization of flowering time between clones in the seed orchard. Flowering of male and female flowers has to occur approximately at the same time in order to fertilize successfully. When selecting plus trees from different regions, the flowering time of the plus trees combined in the seed orchard may differ from each other. If there is only little overlap between the flowering time of male and female flowers, pollen from outside may be more fertile at certain times (Mondal et al. 2019). Therefore, we recommend that a large orchard is established with as many trees as possible. This increases the likelihood that the trees flower synchronously and thus ensures that most pollination will occur within the seed orchard.
In the floodplain forest, only 26.1% of the seeds could be assigned to their fathers, implying that pollen are transported over distances greater than 550 m. However, in the approaches based on paternity analyses, it is often difficult or infeasible to sample all trees in a forest that represent a potential pollen source for reproduction (Bacles and Ennos 2008). Hence, it must be noted that a higher number of parent trees would possibly also shift the mean values, maxima and minima. However, as already discussed, since healthy and only slightly damaged male ash trees (vitality score ≤ 2) contribute more to pollination, there is a chance for gene flow between somewhat isolated individuals or groups of healthy ash trees. Increasingly fragmented ash populations that are expected in the future, as well as already isolated ash trees that are less susceptible to H. fraxineus, may still be in genetic exchange with one another, increasing the chance of a healthier next generation. Individuals of the natural regeneration are the result of natural selection and might be able to withstand inter- or intraspecific competition and could therefore be disease resistant (Metzler et al. 2012). Thus, in the long term, those genotypes that can better withstand the negative effects of the fungus will prevail and have the highest fitness under the prevalent environmental conditions (Fussi et al. 2014). However, Buchner et al. (2022) found that the environmental conditions prevailing during long-distance pollen transport play an important role in the successful pollination of female ash flowers. It was demonstrated that pollen viability decreased faster with increased or prolonged UV radiation and under warmer conditions. Instead, viable pollen could still be observed after 28 days at moderate temperatures. Thus, prolonged heat extremes caused by climate change can have serious consequences for the pollen’s viability during long-distance pollen transport. Nevertheless, it has been shown that gene flow can occur over longer distances, and successful pollination is possible.
In summary, it can be concluded that effective pollen transport occurs over long distances, although with decreasing pollination success. There is still hope for the future of ash since healthy and slightly diseased fathers make a greater contribution to pollination, meaning that gene flow will facilitate the healthier next generation of ash in the future. However, in order to reduce pollen input from outside and avoid cross-pollination on the seed plantations, it is important to establish large seed orchards protected by planting around the border. In addition, female trees should be planted mainly in the center of the orchard to enhance short-distance pollination within the orchard. We recommend harvesting seeds only during masting years due to intensified pollination between plus trees in the orchard.
Availability of data and materials
The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.
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We gratefully acknowledge the Forst Baden-Württemberg and Forstliche Versuchs-und Forschungsanstalt Baden-Württemberg for providing the seed orchard as study site. In addition, we thank Wittelsbacher Ausgleichsfonds for the permission to conduct scientific research in the riparian forest. We thank Tristan Marshall, Johanna Jetschni, Johanna Weidendorfer, and Lisa Thamm for technical assistance.
This research was funded by the Bavarian State Ministry of Food, Agriculture, and Forestry through the Bavarian State Institute for Forests and Forestry (LWF) as part of the project “P035–Quo vadis Pollen? Untersuchungen zur (effektiven) Pollenausbreitung und Pollen- und Samenqualität als Beitrag zur Generhaltung bei der Esche.” Open Access funding enabled and organized by Projekt DEAL.
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Eisen, AK., Semizer-Cuming, D., Jochner-Oette, S. et al. Pollination success of Fraxinus excelsior L. in the context of ash dieback. Annals of Forest Science 80, 22 (2023). https://doi.org/10.1186/s13595-023-01189-5